摘要
【目的】探讨抑癌基因PTEN及DNA错配修复基因hMSH2在年轻妇女子宫内膜癌组织中的表达及相关性。【方法】选取因子宫病变行子宫全切的45岁及以下妇女子宫内膜组织标本66例，其中子宫内膜腺癌42例、子宫内膜非典型增生6例、正常子宫内膜18例。采用免疫组织化学SP法测定PTEN及hMSH2在子宫内膜组织中的表达。【结果】①在正常子宫内膜、子宫内膜不典型增生及子宫内膜癌中PT EN的表达率逐渐降低，缺失率分别为16．67％、66．67％、85．71％，且差异均有极显著性（ P ＜0．01）；hMSH2的表达率分别为44．44％、33．33％、73．81％，各组之间相比较差异有显著性（ P＜0．05）；②PTEN及hMSH2在子宫内膜癌中的表达与临床分期、组织学分级、肌层浸润深度及有无淋巴转移无关（rs 值＝0．271， P ＞0．05）。③在子宫内膜癌中，hMSH2蛋白表达与PTEN 蛋白表达无显著性相关关系（ P ＜0．05）；hMSH2蛋白与 PTEN蛋白表达呈不一致（χ2＝23．04，P＝0．000）。【结论】同时检测PTEN及hMSH2在年轻妇女子宫内膜癌的表达有助于早期诊断，并能推测预后。
[Objective] To explore the expressions of tumor suppressor gene PTEN and DNA mismatch repair gene hMSH2 in young women with endometrial carcinoma and their correlation .[Methods]The endometrial specimens of 66 women（≤45 years old） undergoing total hysterectomy for uterine diseases were collected .Among them ,there were 42 cases of endometrial carcinoma ,6 cases of atypical endometrial hyperplasia and 18 cases of normal endometri-um .The expressions of PTEN and hMSH2 in the tissues of endometrium were detected by immunohistochemical SP method .[Results] The expression rates of PTEN in normal endometrium ,atypical endometrial hyperplasia and endo-metrial carcinoma were decreased gradually ,and the absence rates were 16 .67% ,66 .67% and 85 .71% respectively , and there was significant difference （ P 〈 0 .01） .The expression rates of hMSH2 were 44 .44% ,33 .33% and 73 .81% ,respectively ,and there was significance difference among groups （ P 〈0 .05） .The expressions of PTEN and hMSH2 had no correlation with clinical stage ,histological grade ,myometrial invasion depth and lymphatic metas-tasis whether or not（rs=0 .271 ,P〉0 .05） .There was no significant correlation between hMSH2 and PTEN in en-dometrial carcinoma（ P 〈 0 .05） .The expression of hMSH2 was discordant with the expression of PTEN （χ2 =23 .04 ,P=0 .000） .[Conclusion] The combined detection of PTEN and hMSH2 is helpful for the early diagnosis and can infer the prognosis .
出处
《医学临床研究》
CAS
2014年第3期445-448,共4页
Journal of Clinical Research
基金
湖南省教育厅科学研究项目（编号09C036）
