摘要
在对粳稻‘云引’受稻瘟病菌诱导的近等基因系蛋白质组学分析的基础上,通过实时定量PCR等方法,以普感稻瘟病品种丽江新团黑谷(LTH)为遗传背景,研究含有云引抗稻瘟病菌株"四川-43"基因的近等基因系的抗病和感病材料,预测基因谷胱甘肽转移酶OsGST(Genbank序列号:Os05g0116100)对稻瘟病菌诱导的响应及响应程度的差别。利用RACE技术克隆目的基因cDNA全长序列,并在近等基因系抗病材料的抗病株系和感病株系中进行序列比对,寻找差异位点。结果表明,该基因与短柄二叶草中编码谷胱甘肽转移酶的基因亲缘关系最接近,结合其编码的蛋白质序列分析进一步论证该基因可能作为一个相对保守的抗病防卫相关基因、编码谷胱甘肽转移酶,将基因命名为OsGST。同时构建了该基因的过表达载体、干扰载体,为后续揭示其在感抗材料中响应稻瘟病菌程度差异的原因、研究其功能及抗病机制奠定基础。
Based on the proteomic analysis of blast-resistant near-isogenic lines derived from j aponica rice‘Yunyin’ ,infected with M . oryz ae ,real-time quantitative PCR and other methods were used to explore the blast＆amp;nbsp;resistant and susceptible materials of near-isogenic lines using LTH as genetic background and containing blast‘Sichuan-43’ resistance strain to forecast for rice blast fungus-induced response and the different degrees between the responses of Glutathione S-transferase gene OsGST （Genbank sequence number ：Os05g0116100） .Using RACE technology to get the full cDNA Sequence ,and comparing the sequence between the blast resistant lines and the blast susceptible lines .The phylogenetic tree showed the gene had the closest genetic relationship with glutathione transferase encoding gene of brachypodium distachyon .Combined with the analysis of the protein sequence ,OsGST was suggested to be a conserved gene that encoding glutathione transferase . The overexpression and RNAi expression vector were constructed for subsequent study of its functional identification and resistant mechanisms .
出处
《福建农业学报》
CAS
2014年第3期197-203,共7页
Fujian Journal of Agricultural Sciences
基金
国家"973"计划项目(2010CB723003)
国家转基因农作物新品种培育重大专项(2013ZX08001-002)
福建省财政专项--福建省农业科学院科技创新团队建设项目(CXTD-1-1301)
