摘要
为开发新型天然菌物香料,利用Sepharose CL-6B凝胶柱对二年残孔菌胞外多糖(EPS)进行分离纯化,并利用红外光谱(IR)、气质联用仪(GC/MS)对EPS的特征性结构和单糖组分进行分析。同时,将EPS进行烟草薄片加香实验,采用同时蒸馏萃取和GC/MS分析了试验组与空白组卷烟中香味物质的变化。结果表明,二年残孔菌EPS为单组分多糖,主要单糖组成为葡萄糖51.45%,甘露糖30.30%,半乳糖15.51%。GC/MS分析结果显示,将EPS用于制烟后,烟叶中酮类、醛类和呋喃类的含量增加,酚类、氮杂环类的含量显著下降,这有利于减轻烟气刺激性、改善和修饰卷烟香气,进而提高烟气整体品质。
In order to develop new natural fungal perfume , the fermented extracellular polysaccharides （EPS） from Abortiporus biennis were purified by Sepharose CL-6B.The structure and monosaccharide composition of EPS were investigated by infrared spectroscopy （IR） and GC-MS analysis.After addition of EPS into tobacco conduction , the changes of flavoring substances in cigarettes were compared with control by simultaneous distillation and GC /MS.It was shown that EPS was monocomponent with the monosaccharide composition of 51.45% glucose, 30.30%mannose and 15.51%galactose.With application of EPS in tobacco production , the contents of ketones, aldehydes and furans increased in the cigarettes , while the contents of phenols and heterocyclic compounds decreased significantly.The treatments with EPS could reduce stimulation of smoking , and improve the aroma of cigarette to ameliorate the quality of smoking.
出处
《浙江农业学报》
CSCD
北大核心
2014年第2期279-284,共6页
Acta Agriculturae Zhejiangensis
基金
国家人社部"留学人员科技项目择优支持计划"(豫留学函[2010]16号)
关键词
二年残孔菌
发酵
胞外多糖
香气成分
Abortiporus biennis
fermentation
extracellular polysaccharides
aroma components
