摘要
与酶催化相比,全细胞催化可以避免昂贵、繁琐的酶提取与纯化过程,可以进行涉及多个代谢途径和辅酶再生的生化反应。但是由于细胞膜和细胞壁对底物和产物的传质限制,常导致微生物细胞的催化效率低下。细胞透性化技术可以在不破坏细胞有机整体结构的情况下改善细胞被膜的通透性,使得小分子物质和一些较大分子物质能够自由进出细胞,从而提高细胞催化效率。目前,细胞透性化方法主要有三类方法:透性化试剂添加法、物理处理法和分子生物学法。本文将对微生物细胞的透性化方法与策略做一综述,以期为开展微生物细胞透性化研究提供一些参考。
Using whole cell catalysts have many advantages, such as elimination of the need for tedious, expensive enzyme isolations and purification, and ability to conduct process requiring multiple pathways and cofactor regenerations. But the efficient of whole cell biocatalysis and biotransformation are severely compromised owning to the mass-transfer resistance of the cell wall and membrane towards the substrate and product. So it is important to use cell permeabilization methods to improve cell permeability to enhance cell catalysts efficiency. Cell permeabilization technology can improve cell envelope permeability without total destruction cell integrity. Nowadays, various cell permeabilization methods such as permeabilization agent method, physical treatment and molecular engineering have been developed. This review will introduce the kinds of permeabilization methods and strategies.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2014年第3期125-131,共7页
China Biotechnology
关键词
细胞通透性
微生物
全细胞
生物催化与转化
Cell permeability Microorganism Whole cell Biocatalysis and biotransformation
