摘要
目的建立灵敏、准确的高效液相色谱方法（HPLC）测定大鼠血浆中吉非替尼的浓度，并研究其在大鼠体内的代谢动力学。方法大鼠灌胃或尾静脉注射给予吉非替尼后，自眼球后静脉丛采血，以埃罗替尼为内标物，HPLC法测定血药浓度：甲醇-10mmol／L乙酸铵（70：30）为流动相，流速：1．0mL／min；检测波长：330nm。结果测定吉非替尼的线性范围是0．02016～6．72ug／mL，精密度，稳定性（RSD）均小于15％，口服吉非替尼片绝对生物利用度59．81％。结论该分析方法专属性强，灵敏度高，吉非替尼血浆药物清除曲线符合二室模型。
Objective To establish HPLC for determination of Gefitinib in rat plasma and study its pharmacokinetics characteristics.Methods After a single oral or intravenous injection administra- tion of Gefitinib to each group, blood was collected from the veniplex behind bulbus oculi. Mix- ing Erlotinib as internal standard, HPLC was used to measure the plasma level. The mobile phase consisted of methanol-ammonium acetate solution （ 10 mmol/L） （70 - 30） with the flow rate at 1.0 mL/min and detection wavelength at 330 nm.Results The linear ranges of Gefitinib was 0.020 16-6.72 ug/mL. Precision and stability were less than 15%. Oral bioavailability of Ge- fitinib tablet was 59.81%. Conclusion This analysis method is specific and sensitive, and the plasma drug elimination curve of Gefitinib is concordant with a two compartment model.
出处
《湖北民族学院学报（医学版）》
2014年第1期15-18,共4页
Journal of Hubei Minzu University(Medical Edition)
基金
广东省科技计划项目(2009B030801035)
广东省科技计划项目(2010B030700035)
