摘要
目的 :探讨维拉帕米对大鼠缺血 -再灌流视网膜Glu -IR神经元的作用。方法 :结扎双侧颈总动脉 ,3h后松结复流 ,分别于缺血前 15min和再灌流前 1min腹腔注射维拉帕米 (5mg/kg/次 ) ,对照组腹腔注射等量生理盐水。动物存活 3d ,4%多聚甲醛经心灌注 ,视网膜切片Glu免疫组织化学反应。结果 :正常大鼠视网膜内核层中有大量Glu -IR神经元 ,外核层、节细胞层部分细胞呈Glu -IR。缺血 -再灌流损伤后 ,视网膜内核层中Glu -IR神经元较正常时显著减少 ,染色变淡 ,外核层、节细胞层未见Glu -IR(P <0 .0 5 )。在缺血 -再灌流期应用维拉帕米 ,视网膜中Glu -IR神经元明显增多 ,染色变深 (P <0 .0 5 )。结论 :视网膜Glu -IR神经元对缺血 -再灌流损伤敏感 ,维拉帕米有助于视网膜Glu -IR神经元的存活及免疫活性的恢复。
Objective:To investigation of verapamil on glutamate (Glu) immunoreactivity (IR) neurons of ischemia-reperfusion retina of rat.Methods:Both left and right carotid arteries were clamped for 3h.The carotid arteries of these animals were unclamped.Verapamil(5g/ml for verapamil-treated rats)or equal volume of the vehicle (for untreated animals) was peritoneal injected 15 minutes before 1 minute and after surger.After 3-day survival,the rats were sacrificed.ABC method of immunohistochemistry was used to study the changes of glutamate in retina.Results:Glu-IR neurons distributed the inner nuclear layer,outer nuclear layers and ganglion cell of retina in t he normal rats,After ischemia-reperfusion,Glu-IR neurons decreased and low stained in inner nuclear layer,no Glu-IR neurons in outer nuclear layer and ganglion cell (P<0.05);in verapamil treated animals,Glu-IR neurons increased and high stained (P<0.05).Conclusions:Glu-IR neurons in retina may belong to the sensitive to ischemia-reperfusion,administration of verapamil may benifit the survival and immunoreactivity of Glu-IR neurons in retina.
出处
《中国现代医学杂志》
CAS
CSCD
2001年第2期18-19,共2页
China Journal of Modern Medicine
