摘要
目的:研究蛇葡萄素对大鼠肝星状细胞(HSC-T6)的增殖及胶原蛋白、细胞因子生成的影响。方法:对HSC-T6增殖的影响:用含10%胎牛血清的高糖DMEM培养液制备成HSC-T6细胞3×104个/mL细胞悬液100μL/孔接种于96孔培养板。设细胞对照组、秋水仙碱1.0 mg·L-1组和100.0,75.0,50.0,25.0,12.5 mg·L-15个不同质量浓度蛇葡萄素组,置37℃5%CO2培养48 h。采用MTT法检测蛇葡萄素对大鼠肝细胞HSC-T6增殖的影响。对HSC-T6生成Ⅰ,Ⅲ,Ⅳ型胶原蛋白及细胞因子(TGF-β1,PDGF)含量的影响:取终质量浓度分别为50.0,25.0,12.5 mg·L-1蛇葡萄素,3×104个/mL细胞悬液100μL/孔细胞液接种于96孔培养板。阳性组秋水仙碱的质量浓度为1.0 mg·L-1。置37℃5%CO2培养48 h。ELISA法检测HSCT6细胞生成的胶原蛋白(Ⅰ,Ⅲ,Ⅳ)、转化生长因子(TGF-β1)及血小板衍生因子(PDGF)含量。结果:蛇葡萄素100.0,75.0mg·L-1对HSC-T6细胞的增殖有明显抑制作用,其无毒浓度为50.0 mg·L-1。50 mg·L-1的蛇葡萄素可使HSC-T6细胞生成的Ⅰ,Ⅲ,Ⅳ型胶原蛋白,TGF-β1,PDGF含量明显低于细胞对照组(P<0.05),25 mg·L-1的蛇葡萄素也可使HSC-T6细胞生成的Ⅲ型胶原蛋白含量明显低于细胞对照组(P<0.05)。结论:蛇葡萄素能抑制HSC-T6增殖并可使HSC-T6生成的Ⅰ,Ⅲ,Ⅳ型胶原蛋白,TGF-β1,PDGF含量降低。
Objective：To study the effect of ampelopsin on the HSC-T6 cell proliferation rate and the content of collage and cytokine Method：Effect of ampelopsin on the cell （HSC-T6） proliferation rate.High glucose DMEM medium containing 10％ fetal bovine serum was used to prepare HSC-T6 cells 3 × 104cells/mL suspension 100 μL/well,which was seeded in 96-well culture plates.The control group,colchicine 1.0 mg · L-1 group and 100.0,75.0,50.0,25.0,12.5 mg · L-1,5 different concentrations of ampelopsin groups were designed.Cells were cultured at 37 ℃ 5％ CO2 for 48 h.The cell proliferation inhibition rate was measured by MTT. Effect of ampelopsin on the content of collage （Ⅰ,Ⅲ,Ⅳ） and cytokine （TGF-β1,PDGF） was dected by ELISA.Result：Ampelopsin at 100.0,75.0 mg ·L-1 might effectively inhibit proliferation of HSC-T6 cells,and the non-toxic drug concentration was 50.0 mg · L-1,the contents of collage Ⅰ,Ⅲ,Ⅳ and TGF-β1,PDGF in HSC-T6 cells were significantly lower than control group at 50.0 mg ·L-1 of ampelopsin （P ＜ 0.05）,the contents of collage Ⅲ in HSC-T6 cells was significantly lower than control group by ampelopsin at 25.0 mg· L-1 （P ＜ 0.05）.Conclusion：Ampelopsin could inhibit the proliferation of HSC-T6 and reduce the content of collage Ⅰ,Ⅲ,Ⅳ and TGF-β1,PDGF.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期163-165,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东食品药品职业学院自然科学青年项目(2011YZ009)
