摘要
介绍用羊水细胞直接进行多聚酶链反应的基因分析方法.在妊娠中期,不同孕周(龄)的羊水细胞中有核的羊水细胞数为0.1×10~9/L～0.16×10~9/L.1 ml羊水细胞经处理后,可直接用于20余次PCR,不需提取DNA.在绒毛活检有困难,或孕妇情况不允许,早期产前诊断不能进行时,本法可作为简单、安全、有效的中期产前诊断方法.普通基层医院亦可取材送检,易于推广使用.
A method for gene analysis using amniotic fluid cells directly for PCR(polymerase chain reaction)was described In pregnant women of 16～27th week gestation, there were 0.1×10~9～0.16×10~9/L nucleated cells in the amniotic fluid The amniotic fluid cells were heated in sodium hydroxide solution and used directly for PCR without DNA extraction The cells from 1 ml amniotic fluid were sufficient to perform PCR for more than 20 times.This is a simple, save and reliable method for second trimesterfetal prenatal diagnosis and a complementary means when early fetal prenataldiagnosis failed.It can be applied easily even by hospitals and clinics in the rural areas.
基金
国家自然科学基金及区卫生厅青年科学基金
关键词
聚合酶链反应
产前诊断
基因分析
polymerase chain reaction
gene analysis
second trimester prenatal diagnosis
