摘要
【目的】进一步发掘与大豆产量性状紧密连锁且稳定存在的标记位点,为分子标记辅助选择培育高产大豆新品种奠定理论基础.【方法】利用QTL IciMapping v2.2完备区间作图法连续2年对F2及其衍生群体中4个主要产量相关性状进行QTL定位及效应分析.【结果和结论】以LOD=2.5为阈值,在大豆单株粒数、单株粒质量、百粒质量和单株荚数4个主要产量性状上共检测到19个具有明显加性效应的QTLs,其中主效QTLs 15个,即单株粒数QTLs 3个,单株荚数QTLs 2个,单株粒质量QTLs 10个,分布于4(C2)、12(G)、6(A1)和17(M)4个连锁群上;定位到了3个在2年间稳定存在的QTLs,即单株粒数QTL qNSPP-12-1、单株粒质量QTLs qSWPP-12-1和qSWPP-12-2;研究初步确定了1个新的大豆单株粒质量QTL qSWPP-12-5.研究中检测到的稳定存在和主效QTLs对今后大豆遗传育种研究将具有重要的指导意义.
[Objective]This study explored closely linked and stable marker loci of soybean yield traits , giving a theoretical basis for cultivating high yield soybean varieties by marker -assisted selection .[Method]QTL mapping and effects of four major yield-related traits were analyzed using QTL IciMapping v 2.2 com-plete interval mapping method for two years in soybean F 2 and its derived populations .[Result and conclu-sion]The results showed that the LOD =2.5 was the threshold , and nineteen obvious additive effect QTLs were detected in the number of soybean seeds per plant , seeds mass per plant , 100 seeds mass and number of pods per planting four major production traits , including 15 main effect QTLs , namely , the number of seeds per plant QTLs 3, the number of pods per plant QTLs 2, seeds mass per plant QTLs 10, which were located at 4 （C2）, 12 （G）, 6 （A1） and 17 （M） 4 linkage groups.In addition, three stable QTLs were located in two years , namely, the number of seeds per plant QTL qNSPP-12-1, seeds mass per plant QTLs qSWPP-12-1 and qSWPP-12-2.One new QTL of seeds mass per plant was preliminarily located , namely, qSWPP-12-5.The stable existence and main effect QTLs which were detected in this study will give impor-tant guiding significance for soybean genetics and breeding in the future research .
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2014年第3期41-46,共6页
Journal of South China Agricultural University
基金
吉林省重点科技攻关项目(20140204021NY)
吉林省教育厅科学技术研究项目(2011-41)
吉林省科技厅重点科技攻关项目(20110213)
吉林农业大学校内启动基金(201242)
吉林省科技厅科技引导计划项目(201101111)
关键词
大豆
产量性状
完备区间作图法
QTL定位
SSR
soybean
yield traits
SSR
inclusive composite interval mapping
QTL location
