摘要
【目的】研究饲料中碳水化合物(CHO)与脂肪(L)比例[m(CHO)∶m(L)]对胭脂鱼Myxocyprinus asiaticus幼鱼生长、饲料利用及糖代谢酶活力的影响,确定其适宜添加比例.【方法】以m(CHO)∶m(L)为0.29、0.81、1.58、2.88、5.46和13.22的6种等氮(粗蛋白质量分数41%)等能(16 kJ/g)饲料,投喂初始体质量为(4.01±0.02)g的胭脂鱼幼鱼8周,测定并分析其对胭脂鱼幼鱼增质量率、特定生长率、鱼体营养成分和糖代谢酶活力等指标的影响.【结果和结论】随着饲料m(CHO)∶m(L)增加,胭脂鱼幼鱼的增质量率和特定生长率先上升后下降,均在m(CHO)∶m(L)为5.46时达到最高,并显著高于其他组(P﹤0.05),饲料系数呈相反趋势;全鱼粗脂肪含量随m(CHO)∶m(L)增加显著下降(P﹤0.05),肝脏粗脂肪则呈先降后升的趋势,在m(CHO)∶m(L)为2.88时达到最低;肌肉粗脂肪含量在m(CHO)∶m(L)为13.22时达最低,且显著低于0.29时(P<0.05).随m(CHO)∶m(L)增加,肝脏三酰甘油的含量显著上升(P<0.05),谷草转氨酶和谷丙转氨酶活力先升高后降低,均在m(CHO)∶m(L)为2.88时达到最高;肝脏丙酮酸激酶活力随m(CHO)∶m(L)增加显著上升(P<0.05),而磷酸烯醇式丙酮酸激酶活力则呈下降趋势,己糖激酶和葡萄糖-6-磷酸酶活力无显著变化(P>0.05).用折线回归模型拟合特定生长率和m(CHO)∶m(L)的关系,得到胭脂鱼幼鱼获得最大生长时,饲料中m(CHO)∶m(L)为4.65.
[Objective]This study investigated the influences of dietary carbohydrate-to-lipid m（ CHO）∶m （ L） on growth performance , feed utilization and carbohydrate metabolism enzymes activities of juvenile Chinese sucker , Myxocyprinus asiaticus to detect the suitable addition proportion of m（ CHO）∶m（ L） to the diet of juvenile Chinese sucker .[Method]Six isonitrogenous （ crude protein 41%） and isoenergetic （16 kJ/g） diets were formulated to contain with graded levels of m（ CHO）∶m（ L） （0.29,0.81,1.58, 2.88 ,5.46 and 13.22 ） .Each diet was randomly fed to one treatment of Chinese sucker with an average initial body mass of （4.01 ±0.02） g for 8 weeks.Mass gain rate（MGR）, specific growth rate （SGR）, body composition and carbohydrate metabolism enzymes activities of juvenile Chinese sucker were detec -ted and analyzed .[Result and conclusion]The mass gain rate and specific growth rate increased initially＆amp;nbsp;but then decreased with the increasing dietary m（ CHO）∶m（ L） , thus reaching the highest in fish fed di-ets with m（CHO）∶m（L） of 5.46, which was significantly higher than those of fish fed other diets （P〈0.05）, whereas feed coefficient rates（FCR） showed a converse trend;the crude lipid content in whole-body of fish decreased with the increasing dietary m（CHO）∶m（L） significantly （P〈0.05）, while he-patic crude lipid decreased initially and then increased , reaching the lowest level in fish fed diets with m （ CHO）∶m（ L） of 2.88; Hepatic triglycercide concentrations increased significantly with the increasing dietary m（CHO）∶m（L） （P〈0.05）, while activities of aspartate aminotransferase （AST） and alanine aminotransferase （ ALT ） increased initially and then decreased , reaching the peak with dietary m（CHO）∶m（L） of 2.88;lipid content of muscle was lowest in fish fed diet with m（CHO）∶m（L） of 13.22, which was significantly lower than in fish fed diet with m（CHO）∶m（L） of 0.29 （P〈0.05）.He-patic pyruvate kinase（PK） activities increased significantly with the increasing dietary m（CHO）∶m（L） （ P〈0.05 ） , while phosphoenolpyruvate carboxykinase （ PEPCK ） showed a converse trend .No signifi-cant difference was observed in activities of hepatic hexokinase （ HK） and glucose-6-phosphatase （ G-6-pase ） among dietary treatments （ P〉0.05 ） .Based on broken-line regression analysis of SGR against di-etary m（ CHO）∶m（ L） , a m（ CHO）∶m（ L） of 4.65 was proved to be optimal for the growth of juvenile Chinese sucker .
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2014年第3期1-7,23,共8页
Journal of South China Agricultural University
基金
公益性行业(农业)科研专项(201203086-06)
关键词
胭脂鱼
碳脂比
生长性能
糖代谢酶
Myxocyprinus asiaticus
carbohydrate to lipid level
growth performance
carbohydrate me-tabolism enzyme
