摘要
目的研究曲美他嗪后处理对大鼠急性心肌缺血再灌注后氧自由基损伤及细胞凋亡的影响。方法选择Wistar成年大鼠50只分为假手术组、再灌注损伤模型组(模型组)、曲美他嗪低剂量组(低剂量组)、曲美他嗪高剂量组(高剂量组)、缺血后处理组(后处理组),每组10只。后4组制作缺血再灌注损伤模型后,测定各组大鼠血流动力学变化,超氧化物歧化酶(SOD)、丙二醛水平,光镜、电镜下心肌组织切片观察。结果与假手术组比较,模型组、低剂量组、高剂量组和后处理组左心室舒张末压明显升高,左心室收缩压、左心室内压最大上升和下降速率明显减少(P<0.05,P<0.01)。与模型组比较,高剂量组、后处理组左心室舒张末压明显减低,左心室内压最大上升和下降速率明显增加(P<0.05)。与假手术组比较,模型组、低剂量组、高剂量组、后处理组丙二醛水平明显增高,SOD水平明显降低(P<0.05,P<0.01);与模型组比较,低、高剂量组、后处理组丙二醛水平明显减低,SOD水平明显升高(P<0.05,P<0.01)。结论高剂量曲美他嗪对大鼠心肌缺血再灌注损伤有保护作用。
Objective To study the effect of trimetazidine postconditioning on oxygen free radical jnjury and apoptosis in rats after acute myocardial I/R injury. Methods Fifty Wistar rats were randomly divided into sham operation group, reperfusion injury model group, low trimetazidine dose group, high trimetazidine dose group, and postconditioning group （10 in each group）. A reperfusion injury model was established. The hemodynamics and serum SOD and MDA levels were measured. Myocardial tissue sections were observed under optical and electron microscope, respectively. Results The left ventricular end-diastolic pressure was significantly higher while the left ventricular systolic pressure and the maximal intraventricular pressure were significantly low- er in model group,high trimetazidine dose group, postconditioning group than in sham operation group and significantly lower in high trimetazidine dose group and postconditioning group than in in model group （P^0.01）. The serum level of MDA was significantly higher while that of SOD was significantly lower in model group, low trimetazidine dose group, high trimetazidine dose group and postconditioning group than in sham operation group （P〈0.01）. The serum level of MDA was significantly lower while that of SOD was significantly higher in low trimetazidine dose group, high trimetazidine dose group and postconditioning group than in model group （P〈0.01）. Conclusion High trimetazidine dose can protect rats against myocardial I/R injury.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2014年第4期421-424,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
哈尔滨市科技局科技创新人才研究专项基金(2011RFQYS106)
关键词
曲美他嗪
心肌再灌注
活性氧
细胞凋亡
血流动力学
超氧化物歧化酶
丙二醛
trimetazidine
myocardial reperfusion
reactive oxygen species
apoptosis
hemodynam- ics
superoxide dismutase
malondialdehyde
