摘要
目的通过对人肝癌细胞(HepG2)细胞膜上乙型肝炎病毒(HBV)前S1区(preS1)的结合蛋白进行分离和质谱分析,寻找与HBV黏附相关的特异受体。方法以免疫磁珠法分离与preS1肽段结合的HepG2膜蛋白,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离结合蛋白,选取目的条带进行LC-MS/MS质谱分析及数据库检索。结果 HepG2细胞与preS1结合的蛋白,通过SDS-PAGE电泳分离出了16条带,选取HepG2与preS1结合蛋白分离出重复性较好的6条带,质谱分析出14种蛋白。结论质谱分析的蛋白主要是与物质运输、细胞信号转导、抗原提呈、免疫调节以及能量代谢相关的蛋白。
Objective To seek the specific receptors associated with hepatitis B virus（HBV） adhesion by separating the binding protein of the HBV preS1 region in HepG2 and performing the mass spectrometry. Methods The immunomagnetic bead method was adopted to separate HepG2 membrane protein combined with preS1 peptide fragment and the binding protein was separated by the sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）, then the destination strips was analyzed by LC-MS/MS mass spectrometry and retrieved by the database. Results 16 bands were separated from HepG2 membrane proteins combined with preS1 by SDS-PAGE^14 kinds of proteins were identified from 6 bands with better repeatability separated from HepG2 membrane proteins combined with preS1. Conclusion Protein analyzed by the mass spectrometry is mainly related with the material transport, cellular signal transduction,antigen presentation,immune regulation and energy metabolism.
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第10期1221-1223,共3页
Chongqing medicine
基金
贵州省科学技术基金资助项目[黔科合J字(2008)2157]
贵州省优秀人才省长专项基金资助项目[(2005)297]
关键词
肝炎病毒
乙型
免疫磁珠
乙型肝炎病毒前S1区受体
葡萄糖调节蛋白前体78
hepatitis B virus
immunomagnetic beads
candidate receptor of preS1 of hepatitis B virus
glucose regulated proteinprecursor 78
