摘要
目的研究从杜仲叶中提取的绿原酸对大鼠成骨细胞的促增殖作用。方法MTT法检测分别用0、0．1、1、10μmol/L4种不同浓度梯度的绿原酸和0．1μmol/L绿原酸＋10μmol/LShp2抑制剂NSC87887处理大鼠成骨细胞增殖情况；用无血清无酚红DMEM培养基培养的饥饿大鼠成骨细胞用上述药物处理后，用Western blot检测其p-Shp2（Tyr580）、Shp2、P-Erk和Erk蛋白表达。结果MTT法结果显示绿原酸（0-1μmol/L）促进大鼠成骨细胞的增殖，呈浓度依赖性；Western blot结果显示绿原酸可以诱导大鼠成骨细胞的p-Shp2（Tyr580）和Erk磷酸化水平升高，而NSC87887可以抑制其促进作用。结论绿原酸能够促进大鼠成骨细胞的增值，并且是通过Shp2/Erk途径实现的。
AIM To study the effect of chlorogenic acid extracted from the leaves of Eucommia ulmoides on rat osteoblast proliferation. METHODS MTT method was used to detect rat osteoblast proliferation after being trea ted with four concentrations of chlorogenic acid （0 , 0. 1 , 1, 10 μmol/L） and 0. 1 μmol/L chlorogenic acid ＋ 10 μmol/L Shp2 inhibitor NSC87887. Western blot was used to measure protein expression of p-Shp2 （ Tyr580）, Shp2, p- Erk, and Erk in hunger rat osteoblast incubated in serum-free and phenol red -free DMEM medium. RE SULTS MTT method showed that chlorogenic acid （0 - 1 p, mol/L） promoted rat osteoblast proliferation in a concentration dependent way. Western blot results revealed that chlorogenic acid could induce increased p-Shp2 （Tyr580） and Erk phosphorylation levels of rat osteoblast, but NSC87887 could inhibit that effect. CONCLU- SION Chlorogenic acid can promote rat osteoblast proliferation via the Shp2/ERK pathway.
出处
《中成药》
CAS
CSCD
北大核心
2014年第4期693-697,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金(81160508)
江西省自然科学基金(GZY0170)
