摘要
目的探讨微小RNA（miR）-146a在大肠癌及结直肠息肉患者中的表达及意义。方法收集49例大肠癌患者，37例结直肠息肉患者和30例健康志愿者的血清，应用Taqman荧光定量聚合酶链反应（PcR）方法检测miR-146a在3组中的表达及治疗后的水平，酶联免疫吸附试验（ELIsA）测定癌胚抗原（cEA）水平。分析miR-146a在3组中表达的差异以及大肠癌不同临床病理特征的组间差异。结果大肠癌组miR-146a水平为38．12±3．56，高于结直肠息肉组和对照组，差异有统计学意义（P〈0．05）。在有淋巴结转移的大肠癌患者中nliR-146a表达水平（42．39±5．49）明显高于无淋巴结转移的患者（35．33±6．19，P〈0．05），Dukes分期中的C～D期患者中miR-146a表达水平（41．09±7．11）高于A～B期患者（36．17±0．61，P〈0．05），在不同年龄组及性别组的表达水平上差异无统计学意义（P〉0．05）。治疗后大肠癌细胞miR-146a表达和CEA结果分别为（33．40±2．98）μg/L和（5．32±0．48）μg/L，均低于治疗前的（38．12±3．56）μg/L和（6．68±0．67）μg/L，差异有统计学意义（P〈0．05）。结论miR-146a在大肠癌患者血清中呈高表达，治疗后明显降低，可能在大肠癌的发生、发展中发挥作用。
Objective To investigate the expression and significance of microRNA （miR） -146a in colorectal cancer and colorectal polyps in patients. Methods The study was separated three groups as fol- low： 49 colorectal cancer cases of patients; 37 cases of colorectal polyps patients; 30 healthy volunteers. And then Taqman real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） was used to de tect the expression of miR-146a in the three groups＇ blood serum, and the expression of miR-146a after treatment, carcinoembryonic antigen （CEA） was determinate by Enzyme linked immunosorbent assay （ELISA） and different clinicopathological features of colorectal cancer group differences. Results The colo rectal cancer miR-146a was 38. 12 ± 3.56, higher than that of colorectal polyps group and control group, the difference was significant statistically （P 〈 0. 05）. The lymph node metastasis miR-146a expression levels with colorectal cancer were significantly higher than without lymph node metastasis （ 42. 39 ± 5.49 vs. 35.33 ±6. 19, P 〈0. 05）, Dukes staging of C-D patients miR-146a expression levels higher than A-B pa tients （41.09 ±7. 11 vs. 36. 17 ±0. 61, P 〈0. 05）, no significant difference with different age groups and gender groups above. After treatment, the expression of miR-146a and CEA were （33.40 ± 2.98） μg/L and （5.32 ±0. 48） μg/L, lower than before treatment （38. 12 ±3.56） p.g/L and （6. 68 ±0. 67） μg/L）, the difference was significant （P 〈 O. 05 ）. Conclusion miR-146a was highly expressed in the serum of patients with colorectal cancer and decreased significantly after treatment. It might play a role in the inci dence of colorectal cancer, colorectal cancer development.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第4期862-864,共3页
Chinese Journal of Experimental Surgery
基金
温州市科学技术发展计划项目
