摘要
利用酶促方法合成阿魏酸葡糖酯,对酶和有机溶剂进行了比较和筛选,并对影响阿魏酸葡糖酯产率的因素(酶量、反应时间、反应温度、底物比)进行了研究。结果表明,葡萄糖和阿魏酸乙烯酯(摩尔比4∶1)分别加入到体积比2∶1的无水吡啶和叔丁醇中,20 g/L Novozym 435脂肪酶,在220 r/min的空气振荡器中50℃反应72 h,产率能达到80.9%。
A facile enzymatic approach was developed to synthesize feruloylated glucoside derivatives through lipase-catalyzed transesterification of ferulic acid vinyl ester（FAVE） with glucose. The effect of the various reaction parameters on the synthesis of feruloylated glucoside was studied systematically. Lipase Novozym 435 was found to have the highest catalytic activity in an anhydrous pyridine/tert-butyl alcohol （2：1, volume ratio） solvent mixture. FAVE was selectively conjugated onto the primary hydroxyl group of glucose（ 1：4,mole ratio） ,with a yield of 80.9% under optimal conditions.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2014年第4期475-479,共5页
Fine Chemicals
基金
国家自然科学基金(21303014)~~
关键词
阿魏酸乙烯酯
酶促合成
阿魏酸葡糖酯
食品与饲料用化学品
ferulic acid vinyl ester
enzyme synthesis
feruloylated glucoside ester
food and feedstuffchemicals
