摘要
目的利用线粒体DNA细胞色素B(CYTB)分子标记重新分析流行于青海高原的棘球绦虫基因多态性及系统发育学。方法 PCR方法扩增CYTB基因,测序得到的基因序列碱基通过ClustalX软件比对,构建贝叶斯进化树。结果流行于青海地区的棘球绦虫大部分与细粒棘球绦虫G1型聚在一枝,有四个样本与多房棘球绦虫(AB018440)聚在一枝。结论大部分棘球绦虫基因型属于细粒棘球绦虫G1型,其基因型各不相同,具有多样性。
Objective Partial fragment of cytochrome B（CYTB）gene sequences were used to infer the phylogenetic relationship of Echinococcus spp.Isolates prevailed in Qinghai plateau.Methods CYTB gene segments by PCR,and then sequenced by commercial company.The sequences were aligned using ClustalX1.83 with default data.Bayesian analyses were performed in Mrbayes-3.1.2.Results In both phylogenetic trees,most of Echinococcus spp.isolates belongs to Echinococcus granulosus（G1genetype）except four isolates（Echinococcus multilocularis）while they showed complex phylogenetic relationships among them.Conclusions Further indicating that Echinococcus spp. isolates from Qinghai province may has a more complex evolutionary history than expected.
出处
《青海医学院学报》
CAS
2014年第1期26-30,共5页
Journal of Qinghai Medical College
基金
国家自然科学基金(81360255)
教育部春晖计划(Z2012-082)
关键词
青海
棘球绦虫
系统发育学
细胞色素B
Qinghai
Echinococcus granulosus
phylogeny cytochrome B（CYTB）gene
