摘要
建立了苯酚提取超高效液相色谱法（UPLC）同时测定新鲜烟叶中三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和单磷酸腺苷（AMP）的分析方法。苯酚提取样品溶液中的ATP、ADP和AMP较稳定，不易水解，样品在2 h以内各磷酸腺苷变化率小于10%。采用6‰三乙胺（pH=6.6）和甲醇作为流动相梯度洗脱，3种磷酸腺苷在10 min内实现了较好的分离，线性范围（5～200）μg/mL内，磷酸腺苷浓度与峰面积相关性很高（r〉0.9999），3种磷酸腺苷检测限均小于0.5μg/mL，回收率在91.68%～110.79%之间， RSD在1.53%～3.67%之间。
A analytical method for simultaneous determination of adenosine triphosphate （ATP）, adenosine diphosphate （ADP） and adenosine monophosphate （AMP） in fresh tobacco leaves was established by ultra high performance liquid chromatography （UPLC） with phenol extraction. ATP, ADP and AMP in phenol extraction were stable and difficult to be hydrolyzed with rate of change less than 10%within 2 hours. A better separation of ATP, ADP and AMP was achieved in 10 minutes by utilizing a gradient elution system of 6‰triethylamine （pH=6.6） and methanol as mobile phase. The linear range was between 5~200 μg.mL-1 with adenosine concentration and peak area were highly correlated （r〉0.9999）. The detection limits for ATP, ADP and AMP were 0.5 μg.mL-1, 0.4 μg.mL-1, 0.3 μg.mL-1 respectively, and average recovery rate was 91.68%~110.79%with RSD ranged between 1.53%~3.67%. This method can quickly and accurately detect ATP content in tobacco samples, thus providing important test indicators for the study of energy metabolism, especially tobacco CMS generation mechanism.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2014年第1期26-31,共6页
Acta Tabacaria Sinica
基金
国家烟草专卖局"烟草育种新技术研究及新一轮优质抗病烤烟新品种选育"(合同号:110201002001)
