摘要
目的 观察口腔鳞癌及口腔粘膜高危型人乳头状瘤病毒 (HPV)感染情况 ,探讨HPV感染与临床及病理资料的关系。方法 用多聚酶链反应 (PCR)检测 73例口腔鳞癌及 40例正常口腔粘膜石蜡包埋组织中HPV16、HPV18的DNA。统计分析其与临床及病理资料的关系。结果 口腔鳞癌HPV16 / 18DNA阳性率为 74% (5 4/ 73) ,正常口腔粘膜为 5 5 % (2 2 / 40 )。口腔鳞癌与正常口腔粘膜HPV阳性率存在显著性差异 (P =0 .0 40 )。统计分析显示 :HPV感染与患者的性别、年龄有关 ,与其它因素 (肿瘤发生部位、嗜烟酒情况、肿瘤病理分级、临床分期 )无关。结论 高危型HPV感染与口腔鳞癌的发生有关。口腔粘膜中HPV感染普遍存在 ,提示HPV在口腔肿瘤的发生中并非独立发挥作用。
Objective To ascertain the prevalence of human papillomavirus(HPV) DNA in oral squamous cell carcinomas(OSCCs) and normal oral mucosa and to correlate the virologic data with other factors obtained from patients' records. Methods 113 paraffin embedded tissue blocks including 73 OSCCs and 40 normal oral mucosa were studied by polymerase chain reaction (PCR) for HPV type 16 and 18 DNA. Medical records of patients were reviewed to obtaine the following factors: age, sex, tobacco and alcohol usage, site of tumor, histologic grade and clinical stage. Results 74%(54/73) of OSCCs and 55%(22/40) of normal oral mucosa were HPV 16/18 DNA positive. The presence of HPV DNA showed significant difference between OSCCs and normal oral mucosa ( P =0.040). Statistical analysis indicated that age (60≤years) and sex (male) were associated with the presence of HPV16/18 DNA in the tumor. There were no significant association between HPV presence and other factors, include tobacco and alcohol usage, site of tumor, histologic grade and clinical stage.Conclusions High risk HPV genotypes have a significant association with oral squamous cell carcinoma. It is a co factor in oral carcinogenesis, particularly in male patients and patients under the sixth decade. HPV infection in the oral mucosa is common. HPV is not the single etiological factor responsible for human oral neoplasia. [
出处
《上海口腔医学》
CAS
CSCD
2000年第4期212-215,共4页
Shanghai Journal of Stomatology
基金
国家自然科学基金! (39770 80 )
