摘要
以来自柽柳的耐盐碱基因eIF1A为研究对象,构建其单子叶植物表达载体,并利用农杆菌介导法转化极早熟玉米自交系加2和M1的胚性愈伤组织,创制转eIF1A基因玉米新材料,为耐盐碱转基因玉米育种提供技术和材料支持。结果表明,成功构建eIF1A基因的单子叶植物表达载体pCAMBIA3300-Ubi-eIF1A,共获得33株除草剂抗性植株,其中,12株目的基因和筛选标记基因PCR呈阳性反应,RT-PCR检测表明,目的基因正常转录表达。
The monocot expression vector of salt-tolerant gene elF1A cloned from Tamarix were constructed in this study, and introduced it into embryogenic callus of two very early maturing maize lines M 1 and Jia2 by Agrobac- terium-mediated method. The aim of this study was to create the new maize materials with elFIA gene, for maize breeding. The results showed that monocot expression vector of elF1A gene was constructed successfully named pCAMBIA3300-Ubi-elF1A. There were 33 herbicide resistant plants and 12 of them were positive in PCR and RT-PCR analysis.
出处
《玉米科学》
CAS
CSCD
北大核心
2014年第2期41-45,52,共6页
Journal of Maize Sciences
基金
黑龙江省寒地作物种质改良与栽培重点实验开放课题项目(CGIC201202)
