摘要
建立了荧光增强型量子点探针检测痕量谷氨酸脱氢酶（GLDH）的方法,GLDH是一种烟酰胺腺嘌呤二核苷酸（NAD＋）依赖的酶分子,具有氧化性的NAD＋通过电子转移淬灭CdTe量子点的荧光,而GLDH催化的生物化学反应可以消耗NAD＋.在所采用的NAD＋/GLDH体系中,量子点的荧光先被NAD＋淬灭,加入GLDH消耗NAD＋,荧光会因NAD＋的减少而增强.利用这种高选择性的酶促反应可以检测浓度范围比较宽的GLDH（10～ 1000 U/L）.对于这个浓度范围GLDH的检测在临床上有重要意义,可用于诊断不同类型的肝脏疾病.
We reported a simple and fast fluorescence system based on quantum dots （QDs） to detect glutamate dehydrogenase （GLDH） , which inverted glutamate to α-ketogrutarate using NAD＋ as a coenzyme. The fluorescence of CdTe QDs was quenched by nicotinamide adenine dimucleotide （ NAD＋ ） through an electron transfer pathway, and the quencher NAD＋ could be consumed by adding NAD＋-dependent enzymes and corresponding substrates. Based on this principle we introduced GLDH to consume NAD＋ in the QDs/ NAD＋ system, leading to the enhancement of the fluorescence intensity of QDs, which was in proportional to the amounts of GLDH added. Using this fluorescence system, we measured GLDH in a wide concentration range from 10 U/L to 1000 U/L, which was of significance in clinical diagnosis of different kinds of liver diseases.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2014年第3期436-440,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(Nos.21077108,21173229)
中国科学院方向性项目(No.KJCX2-YW-H29)资助~~
关键词
荧光分析法
量子点
谷氨酸脱氢酶
肝损伤
Fluorescence analysis
Quantum dot
Glutamate dehydrogenase
Nicotinamide adeninedinucleotide
Liver damage
