摘要
目的研究血管内皮生长因子(VEGF)和血管内皮生长因子受体-1(VEGFR-1)在非小细胞肺癌(NSCLC)肿瘤细胞和正常肺组织中的表达情况,探讨VEGF和VEGFR-1在NSCLC发生与发展过程中的意义及两者之间的关系。方法采用免疫组化法,检测40例非小细胞肺癌组织和部分癌旁组织及5例正常肺组织中VEGF和VEGFR-1的表达。采用Log-rank test检测生存率间的差异。采用Cox比例风险回归模型分析临床多因素对生存时间的影响。结果 40例NSCLC组织中,VEGF阳性表达33例,占82.5%;VEGFR-1阳性表达35例,占87.5%。VEGF和VEGFR-1在癌旁组织及正常组织中几乎不表达。在NSCLC组织中VEGF和VEGFR-1的阳性表达率呈正相关(r=0.4428,P<0.01)。VEGF和VEGFR-1在NSCLC细胞中的表达与患者年龄、性别、病理类型、临床病理分期、肿瘤大小、有无淋巴转移均无显著相关(P>0.05)。病理类型和临床病理分期可以作为独立危险因素影响NSCLC术后生存时间,而VEGF、VEGFR-1的表达等其他特征作为独立危险因素影响NSCLC术后生存时间尚无统计学差异(P>0.05)。结论本研究中VEGF和VEGFR-1在NSCLC细胞胞浆中表达,VEGF可通过VEGFR-1直接作用于NSCLC细胞。VEGF和VEGFR-1在NSCLC细胞中的表达与肿瘤组织生物学性质和预后无明显相关。
Objective To study the expressions of VEGF and VEGFR-1 in the tissue of NSCLC, paracancerous and normal tissues, and discuss their significances in the genesis, growth of NSCLC and the relationship between them. Methods By using the method of immunohistochemistry, the expressions of VEGF and VEGFR-1 were detected in 40 cases of NSCLC, part of paracancerous tissues of NSCLC, and 5 cases of normal lung tissues. The log-rank test was used to determine statistical differences between survival rates. Cox＇s proportional hazards regression model was used to analyze the effect of clinical variables on overall survival. Results Among 40 cases of NSCLC, 33（82.5%） cases and 35（87.5%） cases were positive expressions of VEGF and VEGFR-1 in NSCLC, respectively. There was positive proportion between VEGF and VEGFR-1 expression in NSCLC（r=0.4428, P〈0.01）. VEGF and VEGFR-1 expressions in NSCLC were not statistically correlated with patients＇ age, gender, pathologic classification, clinical and pathologic staging, tumor＇s size, lymphatic invasion. （P〉0.05）. As independent risk factors, pathologic classification, clinical and pathologic staging affected postoperative survival time, while there was not statistical difference between the effects of VEGF expression, VEGFR-1 expression and other characters on postoperative survival time in this study（P〉0.05）. Conclusion VEGF and VEGFR-1 express in the plasma of NSCLC cells. VEGF can directly act on NSCLC cells via VEGFR-1. There was no relationship between the expression of VEGF and VEGFR-1 in NSCLC cells and biology character/prognosis in this study.
出处
《中华临床医师杂志（电子版）》
CAS
2013年第24期151-153,共3页
Chinese Journal of Clinicians(Electronic Edition)
关键词
癌
非小细胞肺
血管内皮生长因子A
血管内皮生长因子受体1
Carcinoma, non-small-cell lung
Vascular endothelial growth factor A
Vascularendothelial growth factor receptor-1
