摘要
目的检测肝细胞癌(HCC)组织中miR-122和上皮性钙粘蛋白(E-cadherin)、转化生长因子-β1(TGF-β1)的表达,并分析miR-122与E-cadherin、TGF-β1之间的相关性。方法应用实时荧光定量PCR技术检测45例HCC及其配对癌旁组织中miR-122的表达情况;免疫组织化学法检测上述HCC及其配对癌旁组织中E-cadherin、TGF-β1的表达,分析miR-122的表达量与E-cadherin、TGF-β1之间的相关性。结果 miR-122在肝癌组织低表达,肝癌组织中miR-122表达量低于其癌旁组织者有41例,表达量相仿或高于其癌旁组织者4例,差异具有统计学意义(P<0.05)。E-cadherin在HCC组织中的表达显著低于癌旁组织(P<0.05),HCC组织中TGF-β1表达显著高于癌旁组织(P<0.05)。HCC组织中miR-122表达与E-cadherin呈正相关(r=0.827),与TGF-β1表达呈负相关(r=-0.356)。结论 miR-122在HCC组织中低表达,其表达量与肝癌分化程度正相关。
Objective To detect the expression of microRNA-122 （miR-122）, E-cadherin（E-cad） protein, transforming growth factor-β1（TGF-β1） protein and analyze the correlation between the expression of miR-122 and E-cad, TGF--β1 in the samples of patients with HCC. Methods The quantitative reverse transcription-PCR was used to detect the expression of miR-122 in 45 patients with HCC and the immunohistochemical method was used to detect the expression of E-cadherin and TGF-β1 protein in the tissues of HCC patients.The correlation between the miR-122 and the E-cadherin,TGF-β1 was analyzed. Results The expression of miR-122 was low in HCC and the expression of tumor tissues was lowed than side-tumor tissues in 41 patients with HCC and there were significant differences between them（P〈0.05）.The expression of E-cadherin was significantly lower in tumor tissues and the side-tumor tissues（P〈0.05）. The expression of TGF-β1 was significantly higher in tumor tissues and the side-tumor tissues（P〈0.05）. It showed positive correlation between the miR-122 expression and the E-cadherin expression and it showed negative correlation between the miR-122 expression and the TGF-β1 expression. Conclusion The expression of miR-122 in the patients with HCC was low and correlated positively with the tumor differentiation of HCC.
出处
《中华临床医师杂志（电子版）》
CAS
2013年第24期131-133,共3页
Chinese Journal of Clinicians(Electronic Edition)
基金
云南省应用基础研究计划项目(2001FB159)
