摘要
目的:建立过岗龙片的薄层鉴别方法。方法:以3’,4’,5’,5,7-五甲氧基黄酮和3’,4’-亚甲二氧基-5’,5,6,7-四甲氧基黄酮为对照品,对过岗龙片进行薄层鉴别,并考察不同展开系统、不同点样量、不同薄层板、薄层板不同厚度、不同检视方式、不同CMC-Na浓度、不同温度、不同湿度对过岗龙片薄层色谱的影响。结果:以3’,4’,5’,5,7-五甲氧基黄酮和3’,4’-亚甲二氧基-5’,5,6,7-四甲氧基黄酮为对照品,石油醚-乙酸乙酯(2:5)为展开系统,过岗龙片薄层鉴别特征明显,专属性强。结论:该方法可行性好,可作为过岗龙片的薄层鉴别方法。
Objective：To establish a TLC identification method for tablets of guoganglong. Methods：With 3＇,4 ＇-methyLenedioxy-5 ＇, 5,6,7-tetramethoxyfLavone and 3 ＇, 4 ＇ , 5 ＇, 5,7-pentamethoxyfLavone as the reference substances, different development system, different spotting sample volume, different thin chromatography plate, different thickness of the thin Layer plate, different inspection, different concentration of CMC-Na, different temperature and different humidity were investigated. Results：With 3＇, 4 ＇-methyLenedioxy-5 ＇, 5,6,7-tetramethoxyfLavone and 3 ＇, 4 ＇, 5 ＇, 5,7- pentamethoxyflavone as the reference substances, TLC i- dentification for tablets of guoganglong was high specificity with Petroleum ether - ethyl acetate（2..5） as the development system. Conclusion：The method established for tablets of guoganglong is feasible and reproducible, and can be used as tablets of guogan- glong identification.
出处
《亚太传统医药》
2014年第6期33-36,共4页
Asia-Pacific Traditional Medicine
