摘要
【目的】观察穿山龙水溶性总皂苷对体外培养类风湿关节炎患者成纤维样滑膜细胞核因子κB(NF-κB)p65及其抑制因子IκB-α蛋白水平和mRNA含量的影响。【方法】培养类风湿患者成纤维样滑膜细胞,分为穿山龙水溶性总皂苷低、中、高剂量组(中药低、中、高剂量组,10、20、30 mg/L),模型组,同时设立空白对照组;经药物干预后,采用Western blot法测定NF-κB和IκB-α蛋白水平,采用半定量逆转录—聚合酶链反应(RT-PCR)方法检测成纤维细胞中NF-κB和IκB-αmRNA的表达。【结果】经穿山龙干预后的关节炎患者成纤维样滑膜细胞的NF-κB活化受到显著抑制,蛋白及基因表达水平较模型组显著下降(P<0.05),IκB-α蛋白及基因表达显著提升(P<0.05)。【结论】穿山龙水溶性总皂苷可以抑制类风湿关节炎患者成纤维样滑膜细胞的核转录因子的表达,这可能是穿山龙治疗类风湿关节炎的机理之一。
Objective To observe the effect of water-soluble total saponin of Rhizoma Dioscoreae Nipponicae （RDN） on protein levels and mRNA contents of nuclear factor-kappa B （NF-KB） p65 and its inhibition factor IKB-α in fibroblast-like synoviocytes of rheumatoid arthritis patients in vitro. Methods Fibroblast-like synoviocytes of rheumatoid arthritis patients were cultured, and then were divided into four groups, namely 10, 20 and 30 mg/L water-soluble total saponin of RDN groups, model group and blank control group. The levels of NF-KB and IKB-α protein were assessed by Western blotting method and their mRNA expression levels were measured by semi-quantitative RT-PCR method. Results The activation of NF-KB in fibroblast-like synoviocytes was significantly inhibited in the three RDN groups , and the protein and gene levels of NF-KB were obviously decreased （P〈0.05, compared with those in the model group） , while the protein and gene levels of lKB-α were increased （P〈0.05）. Conclusion Water-soluble total saponin of RDN can effectively inhibit the expression of nuclear transcription factor in fibroblast-like synoviocytes of rheumatoid arthritis patients, which may be one of the therapeutic mechanisms of RDN for rheumatoid arthritis.
出处
《广州中医药大学学报》
CAS
北大核心
2014年第2期243-246,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:30873420)
河北省教育厅指令课题(编号:2011163)
河北省高等学校科学技术项目(编号:ZH200806)
承德市科技局指导课题(编号:201121024)
关键词
类风湿关节炎
中药疗法
穿山龙水溶性总皂苷
药理学
成纤维样滑膜细胞
基因表达调控
蛋白表达
细胞培养
Rheumatoid arthritis/TCD therapy
Water-soluble total saponin of Rhizoma Dioscoreae Nipponicae/pharmacology
Fibroblast-like synoviocytes
Gene expression regulation
Protein expression
Cell culture
