摘要
本试验旨在研究热应激对奶山羊瘤胃黏膜紧密连接蛋白表达的影响。将8只体况良好,体重、年龄、胎次和产奶量相近,处于泌乳中后期的奶山羊随机分为对照组和热应激组,每组4只羊。饲养环境采取人工控温控湿。试验期45 d,在试验30和45 d时,采集瘤胃背囊和腹囊组织,分别采用免疫组化法、实时定量PCR法和蛋白质免疫印迹法检测其闭合蛋白(claudin)、闭锁小带蛋白1(ZO-1)蛋白的结构分布、基因mRNA表达量和蛋白表达量。结果表明:与对照组相比,热应激组(30 d)背囊和腹囊中claudin、ZO-1蛋白的信号强度和表达量均有所下降,而热应激组(45 d)下降更为严重。与对照组相比,热应激组(30 d)背囊和腹囊中claudin、ZO-1基因mRNA表达量均显著下降(P<0.05),而热应激组(45 d)则显著低于热应激组(30 d)(P<0.05)。结果提示,热应激对瘤胃黏膜的结构和完整性的破坏与瘤胃黏膜紧密连接蛋白表达量下降有关。
This study was conducted to study the effects of heat stress （ HS） on expressions of tight junction proteins in ruminal mucosa of dairy goats. Eight healthy dietary goats with similar body weight, age, parity and milk yield at mid to late-locating period were randomly divided into control group and heat stress （ HS ） group with 4 goats in each group. Environmental temperature and humidity during feeding period were artifi-cially controlled. The experiment lasted for 45 days. Knapsack and abdominal sac samples were collected at 30 and 45 days of experiment to determine protein structural distribution, gene mRNA expression levels and pro-tein expression levels of claudin and zonula occluden 1 （ ZO-1 ） using the methods of immunohistochemisty, real-time quantitative PCR and West-bloting, respectively. The results showed as follows： compared with the control group, expression levels and signal intensity of claudin and ZO-1 proteins in knapsack and abdominal in HS group （30 days） tened to be decreased, and those in HS group （45 days） were further decresed. Com-pared with the control group, mRNA expression levels of claudin and ZO-1 genes in knapsack and abdominal in HS group （30 days） were significantly decreased （P〈0. 05）, and those in HS group （45 days） were sig-nificantly lower than those in HS group （30 days） （P〈0. 05）. In conclusion, the damage of heat stress on structure and integrity of ruminal mucosa is due to the declined expression of tight junction proteins.
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第3期768-775,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
公益性行业(农业)科研专项(200903003)
内蒙古农牧业科学院青年创新基金(2012QNJJM01)
关键词
热应激
奶山羊
瘤胃黏膜
紧密连接蛋白
heat stress
dairy goats
ruminal mucosa
tight junction protein
