摘要
采用ＥＭＳ 处理水稻体细胞愈伤组织诱导变异，试验表明，采用浸渍法处理２ｈ ，ＥＭＳ 对水稻体细胞愈伤组织的半致死质量浓度为１ ～５ ｇ／Ｌ；采用滴加法处理，每管加０ ．１ ｍｌ 时，半致死浓度为５０ ｇ／Ｌ左右。根据出苗率和变异情况，用滴加法处理，每管滴加０ ．２ ｍｌ 时，处理浓度以１ ｇ／Ｌ为好。经ＥＭＳ 处理后，再生植株株系的变异频率比对照高５ ．０２ ％ 。
The method and optimum concentration that EMS was used to treat rice somatic callus for mutant induction and some mutant lines were obtained. The result showed that the concentration of 1～5m g/L 50%LD of EMS was used to soak rice somatic calli for two hours; and in dropping method, the concentration was 1～5m g/L but 0.1ml EMS solution was added on the surface of callus every tube. According to the result of mutation of generation the concentration of 1m g/L was appropriate. The mutation ratio of generation was significantly higher than check after EMS treatment.
出处
《安徽农业科学》
CAS
1999年第6期528-530,共3页
Journal of Anhui Agricultural Sciences
