摘要
运用生物短期试验 (Ames试验 )探讨了柴油机排气微粒的直接致突变性 ,并对发动机不同工况下微粒的直接致突变性变化进行了评估。所用的菌种为 TA98和 TA10 0 ,主要考察碱基对置换突变和移码致突变。试验结果表明 ,柴油机排气微粒 Ames试验显阳性 ,具有强烈的致突变性 ;菌落回变数同柴油机微粒质量及排气体积线性关系不明显 ;除了多环芳香烃外 ,还有其它有机物质对柴油机直接致突变性影响很大 ;大气微粒的单位质量致突变性同柴油机相当 ,而单位体积的致突变性 。
WT5”BZ] Using Salmonella typhimurium plate incorporation(Ames) assay,the mutagenicity of diesel exhaust particulates under various test modes was studied.In Ames assay the strains were TA98 and TA100,which were able to determine base pair substitutions and frameshift mutation respectively.The experimental results were that diesel exhaust particulates showed positive effect in Ames assay and strong mutagenicity and not all of the revertants of strains were linear with diesel particulate mass and exhaust volume.Besides polycyclic aromatic hydrocarbons,there were other direct acting mutagens.The revertants per unit mass of ambient air particulates were equal to that of diesel exhaust particulates,while the revertams per unit volume of diesel exhaust were nearly 10 000 times as high as that of ambient air. [WT5”HZ]
出处
《内燃机学报》
EI
CAS
CSCD
北大核心
2000年第4期375-379,共5页
Transactions of Csice
基金
国家自然科学基金资助项目! (597760 2 6)
关键词
柴油机
致突变性
排气微粒
生物短期试验
Diesel exhaust particulate
Mutagenicity
Ames test
Salmonella typhimurium
