摘要
目的评价丹参酮ⅡA磺酸钠对2型糖尿病大鼠心肌肿瘤坏死因子(TNF)-αmRNA表达的影响,探讨其对糖尿病心肌病的保护机制。方法将大鼠随机分为正常组、模型对照组及丹参酮ⅡA磺酸钠组。成模后丹参酮ⅡA磺酸钠组给药12周,留取血及心肌标本用放免法检测各组大鼠血浆TNF-α,RT-PCR方法检测心肌组织中NF-κB、TNF-αmRNA表达水平。结果模型对照组大鼠血浆中TNF-α水平较正常对照组明显升高(P<0.01),且心肌组织中NF-κB、TNF-αmRNA表达较正常对照组明显增加(P<0.01)。给药12周后,丹参酮ⅡA磺酸钠组心肌组织中NF-κB、TNF-αmRNA表达较模型对照组明显降低,两组之间差异显著(P<0.05)。结论丹参酮ⅡA磺酸钠可能通过抑制2型糖尿病大鼠心肌组织中NF-κB通路,减少TNF-αmRNA表达,对2型糖尿病大鼠心肌提供保护作用。
Objective：To evaluate the effect of tanshinone Ⅱ A sulfonate sodium on TNF-α mRNA expressions in myocardium of rats with type-Ⅱ diabetics and further discuss the protective mechanism of diabetic cardiomyopathy.Methods：Wister rats were randomly divided into normal control group （NC）,model control group （DM） and tanshinone Ⅱ A sulfonate sodium treatment group （DMS）.After rats model was established and STS was administrated for 12 weeks.Specimens of blood and myocardium in rats were reserved for detecting serum TNF-α by radio immunoassay and expressions of NF-κB （Nuclear factor-kappa B） 、TNF-α mRNA in myocardium by RT-PCR.Results：Compared with the NC group,the level of TNF-α in DM group elevated （P＜ 0.01）.Compared with the NC group,the NF-κB and TNF-α mRNA expressions in myocardium of DM group significantly increased （P＜ 0.01）.However,the NF-κB and TNF-α mRNA expressions in myocardium of DMS group after 12 weeks treatment were less than those of DM group,showing statistical differences （P＜ 0.05）.Conclusion：Tanshinone Ⅱ A sulfonate sodium can reduce TNF-α mRNA expressions in myocardium of rats with type-Ⅱ diabetics by inhibition of NF-κB pathway and protect the diabetic rats＇ myocardium.
出处
《中国中医急症》
2014年第3期440-441,454,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
广东省江门市科技局资助课题(20107514)
