摘要
用不同产地和采收期的十批周方提取物建立HPLC指纹图谱,同时进行对照品比对;选道地产地的周方提取物为标准图谱,比较分析相似度法与主成分分析、聚类分析及主成分-聚类这三种化学模式识别方法对质量评价的差异。结果表明:指纹图谱共32个共有峰,其中共有峰5、6、9、16、17、18、20、21、24、27分别为儿茶素(Catechin)、绿原酸(Chlorogenic acid)、羟基红花色素A(Hydroxysafflor yellow A)、芦丁(Rutin)、金丝桃苷(Hyperin)、木犀草素-7-O-β-D-葡萄糖苷(Luteolin-7-O-β-D-glucoside)、槲皮苷(Quercitrin)、芹菜素-7-O-β-D-葡萄糖苷(Apigenin-7-O-β-Dglucoside)、黄芩苷(Baicalin)、槲皮素(Quercetin);单独运用指纹图谱相似度方法难以全面体现中药复方质量的差异,而结合主成分分析、聚类分析及主成分-聚类分析这三种化学模式识别方法,可以从不同角度更客观地评价中药复方质量。
The ten batches of Zhou＇ s prescription extract that contains herbs that were collected from different cultivation areas and at different harvest times, and their HPLC fingerprint were established and simultaneously compared with standard reference.Zhou＇ s prescription extract,which were obtained from Daodi cultivation areas, were selected as the standard chromatogram. The three chemometrics methods including hierarchical cluster analysis（HCA）, principal component analysis （PCA）and principal component-hierarchical cluster analysis （PC-HCA）were used to compare difference of quality evaluation with similarity.Results showed that 32 peaks were selected as the common peaks in fingerprint, and common peak 5,6,9, 16,17, 18,20,21 ,24 and 27 was Catechin, Chlorogenic acid, Hydroxysafflor yellow A, Rutin, Hyperin, Luteolin-7-O-β-D-glucoside, Quercitrin ,Apigenin-7-O-β-D-glucoside, Baicaiin and Quercetin, respectively.It was difficult to reflect comprehensively the difference of Chinese herbal compound prescription by using the fingerprint similarity, and the above three chemometrics methods can evaluate objectively the quality of Chinese herbal compound prescription from various angles.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第7期296-302,共7页
Science and Technology of Food Industry
基金
国家"十一五"科技支撑计划(2006BAI11B08-1)
江苏省社会发展项目(BE2011848)
江苏省"六大人才高峰计划"(10-yy-078)
关键词
周方
指纹图谱
化学计量学
质量评估
.Zhou＇ s
fingerprint
chemometrics
quality evaluation
