摘要
以石油醚为提取剂、料液比为1∶7,进行单因子和正交试验及急性毒性实验。结果表明:超声波辅助提取绞股蓝种子油的最适工艺条件为提取的温度60℃、功率200 W和时间50 min,此条件下种子油提取率为28.13%;绞股蓝种子油的密度为0.895 g·mL-1、折光值1.518 n D20、酸值0.85和皂化值180.2 mg KOH·g-1、碘值135.7 g I2·100g-1,为优良的干性油;绞股蓝种子油含有10种脂肪酸成分,主要为亚麻酸(80.49%)、油酸(8.05%)、亚油酸(7.08%)、硬脂酸(1.79%)和棕榈酸(1.28%),不饱和脂肪酸质量分数达总脂肪酸的96.48%;以剂量65 mL·(kg·d)-1的绞股蓝种子油灌胃小鼠,在连续14 d的实验期内,没有发现小鼠的中毒症状和死亡现象。初步判定绞股蓝种子油无急性毒性,有望作为营养保健油源进一步开发。
Based on petroleum ether as extraction solvent and ratio of material to solution 1∶7,the ultrasonic-assisted extraction process on seed oil of Gynostemma pentaphyllum was optimized by single factor test and orthogonal test. The optimal ultrasonic-assisted conditions were as follows：extraction temperature 60 ℃,ultrasonic power 200 W and extraction time 50 min,under which the extraction rate of seed oil could reach 28.13%.Physicochemical properties of the seed oil including density,dioptre,acid value,saponification value and iodine value were respectively 0.895 g·mL-1,1.518 nD20,0.85,180.2 mg KOH·g-1 and 135.7 g I2·100g-1,which revealed thatG.pentaphyllum seed oil was a fine drying oil.GC-MS analysis revealed that there were 10 kinds of fatty acids in G.pentaphyllum seed oil,and the mass fraction were linolenic acid（80.49%）,oleic acid（8.05%）,linoleic acid（7.08%）,stearic acid （1.79%）and palmitic acid（1.28%）.The unsaturated fatty acids accounted for 96.48% of total fatty acids.In the acute toxicity test,a dose of 65 mL·（kg· d）-1 of seed oil were filled into mice’s stomach,and no evident poisoning symptoms and animal death were detected after 14 d continuous observation,which preliminarily indicated that G. pentaphyllum seed oil was of nontoxicity,and expected to be developed as a resource of health oil.
出处
《广西植物》
CAS
CSCD
北大核心
2014年第1期130-134,共5页
Guihaia
基金
国家自然科学基金(31260039)
湖南省科技计划项目(2012NK3100)
湖南省高校产业化培育项目(10CY015)
关键词
绞股蓝
种子油
理化性质
脂肪酸组成
急性毒性实验
Gynostemma pentaphyllum
seed oil
physicochemical property
fatty acid composition
acute toxicity
