摘要
利用野生稻有利基因是培育超高产栽培稻 ( Oryza sativa L,2 n=2 4 AA染色体组型 )的主要技术路线之一。本研究通过穗茎注射法将小粒野生稻 O.minuta( 2 n =4 x=4 8,BBCC染色体组型 )总DNA导入杂交稻当家亲本 V2 0 B,获得了变异株的 3个高世代株系 ;用 RAPD方法对上述 3个株系、供、受体及对照品种明恢 63共 6个材料进行了多态性研究分析。所用 83个 Operon引物均在小粒野生稻与V2 0 B间扩增出多态性产物 ,二者相似系数仅为 2 6.7% ,而明恢 63与 V2 0 B二者的相似系数达 90 .5%。83个引物中有 15个引物在变异株第 7代株系 97C- 77与受体 V2 0 B间扩增出的产物存在差异 ,差异带共有 2 6条 ,占 83个引物在 V2 0 B中扩增出的带数 397的 6.6%。其中 OPB- 18、OPD- 0 3、OPE- 0 9、OPE- 18、OPG- 10及 OPG- 11等 6个引物在 97C- 77中各扩增出 1条只在供体中存在而在受体中不存在的特异带。 2 6条差异带中的 2 4条在共第 3代祖先的第 9代 2个株系 98C- 10 7、 98C- 165与 97C- 77间无差异。因此 ,绝大多数 DNA水平上的变异可能从第 3代起稳定遗传 ;研究结果从 DNA水平证明 ,变异株系中导入了野生稻 DNA,导入野生稻
Utilization of favorable genes from wild rice is a promising way for rice super high yield breeding. Transferring those genes into cultivars is the first and key step. Total DNA of O.minuta (2n=48) was isolated and injected into the spike stalks of V20B (an elite maintainer line of hybrid rice) during suitable period. Variants were found in the first generation. From these variants new maintainer line have been developed. RAPD analysis with 83 Opron primers was performed among Minghui 63 (control)、 O.minuta (donor)、 V20B(recipient)、 97C 77 (a strain of the 7th generation from a variant)、 98C 107 and 98C 165 (two strains of the 9 th generation from sister strains of 97C 77). Products of each primer were detected to have polymorphism between V20B and O.minuta. Products of 15 primers are different between 97C 77 and O.minuta. but not in V20B and Minghui 63. Only 2 of them have polymorphism among 98C 107、 98C 165 and 97C 77, the others are identical. These results indicated that the method of injecting wild rice DNA into cultivars spikestalk was an effective method to transfer favorable genes from distant relatives besides gene engineering method and interspecific cross method aided by embryo rescue.
出处
《作物学报》
CAS
CSCD
北大核心
2000年第4期424-430,共7页
Acta Agronomica Sinica
基金
"8 6 3"水稻生物技术项目
总理基金项目
湖南省科委的支持
