摘要
目的调查青岛地区大肠埃希菌临床分离株中β-内酰胺酶基因blaCTX-M和质粒介导的喹诺酮耐药(Plasmid-mediated quinolone resistance,PMQR)基因的流行性。方法采用Vitek-2compact系统进行菌株鉴定和药物敏感性测定。聚合酶链反应(Polymerase chain reaction,PCR)法检测菌株中存在的blaCTX-M基因和各种PMQR基因,并对PCR扩增产物进行DNA序列测定,确定基因类型。结果 83%的大肠埃希菌对头孢菌素类和氟喹诺酮类药物耐药。84%的大肠埃希菌带有blaCTX-M基因,38%、23%、3%、6%和5%的大肠埃希菌分别带有aac(6’)-Ib-cr、oqxAB、qnrB、qnrS及qepA基因。60株菌同时含有PMQR和blaCTX-M基因,其中10株菌同时含有两种PMQR基因及blaCTX-M基因,3株菌同时含有三种PMQR基因及blaCTX-M基因。结论 blaCTX-M基因和PMQR基因在青岛地区大肠埃希菌临床分离株中的流行性较高,且常共存于同一株细菌中,这将促进该地区临床细菌多重耐药性产生,加重细菌性感染治疗的难度。
Objective This study aimed to investigate the prevalence of β-1actamase gene blacTx M and plasmid-mediated quinolone resistance （PMQR） genes in clinical Escherichia coli isolates from Qingdao,China.Methods Strain characterization and antibiotic susceptibility analysis were performed by using Vitek-2 compact system.The presence and types of blaCTX-M and PMQR genes in clinical isolates were determined by polymerase chain reaction （PCR） and DNA sequencing.Results 83％ isolates could be co-resistant to cephalosporins and fluoroquinolones.84 ％ isolates contained the blacTX-M gene.Prevalences of the PMQR genes were：aac （6 ＇）-Ib-cr,38 ％ ; oqxAB,23 ％ ; qnrB,3 ％ ; qnrS,6 ％ and qepA,5 ％.60 isolates simultaneously carried both PMQR and blacTx-M genes,10 carried two PMQR genes and blaCTX-M genes,and 3 carried three PMQR genes and blacTx M genes.Conclusion High prevalence of PMQR and blaCTX-M genes were identified in clinical E.coli isolates from Qingdao,which would promote multidrug resistance widespread in clinical settings and aggravate the difficulty of bacterial infection treatment in Qingdao.
出处
《中国实验诊断学》
2014年第3期425-428,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金项目(31100059)
中央高校基本科研业务费专项资金(10CX04021A)
青岛经济技术开发区重点科技发展计划项目(2013-1-82)
