摘要
目的检测深圳市宫颈癌患者癌组织中HPV16型E6、E7致癌基因的序列。方法采用PCR技术扩增1例在深圳生活17年宫颈癌HPV16型阳性患者组织中HPV16E6、E7基因,对其进行克隆、构建重组质粒并进行核酸限制性内切酶鉴定及DNA测序分析。结果成功地克隆了HPV16E6、E7基因,测序分析表明克隆的HPV16E6、E7基因与GenBank收录的德国标准株相比完全一致。结论深圳市妇女宫颈癌组织中HPV16型E6、E7致癌基因的序列与德国标准株相同。
Objective To investigate the coding sequences of HPV16 E6/E7 gene extracted from the cervical cancer tissues from patients in Shenzhen.Methods The HPV16 E6/E7 gene was extracted from a HPV16 positive cervical cancer patient that had been living in Shenzhen for 17 years.The gene was amplified by PCR and cloned;the recombinant plasmid was constructed;and the DNA Sequence was restricted by restriction endonuclease.Results The HPV16 E6/E7 gene was successfully cloned and the sequencing analysis showed that the cloned HPV16 E6/E7 gene is identical to the German standard strain documented in GenBank.Conclusion The coding of HPV16 E6/E7 gene extracted from cervical cancer tissues from female patient in Shenzhen is identical to the German standard strain.
出处
《中国实验诊断学》
2014年第3期397-400,共4页
Chinese Journal of Laboratory Diagnosis
基金
深圳市科技局立项课题(医疗卫生类)(201003042)
关键词
宫颈癌
人乳头瘤病毒
E6、E7致癌基因
序列分析
cervical cancer
human papillomavirus （HPV）
E6 oncogene, E7 oncogene
sequence analysis
