摘要
利用原代鸭胚成纤维细胞培养及蚀斑克隆技术从具有典型渗出性、坏死性病变的雏鹅新型病毒性肠炎 (NGVE)病例的实质器官和肠道内容物中分离到 2 6株病毒。经鉴定 ,2 6株病毒的中和性抗原是一致的。病毒 (NGVEV)呈球形或椭圆形 ,无囊膜 ,直径 70～ 90nm。不凝集鸡、鸭、鹅、鸽、黄牛、水牛及猪的红细胞。对氯仿处理 1～ 3次不敏感。病毒于 - 15℃和 0℃至少可以分别保存 36和 2 0个月 ;于 37℃ 4 5d、4 5℃ 4 8h、56℃ 5h、60℃ 1h不影响病毒对细胞的致病变能力和对雏鹅的致病性 ;于 80℃ 5min和煮沸 ( 96℃ ) 10s可以使病毒失活。病毒经药物抑制法和酶消化法均鉴定其核酸类型为DNA。在CsCl中浮密度为 1 32 g/cm3。pH1 0和pH10 0处理 1h可使病毒失活 pH2 0和 9 0可使病毒滴度有所下降 ,pH3 0～ 8 0对病毒的感染性没有影响。NGVEV与鸭瘟病毒和小鹅瘟病毒没有中和抗原相关性。分离的病毒通过滴鼻、点眼、皮下注射、肌肉注射、口服等途经人工感染均可复制出与临床表现一致的病例 ,其中口服是最佳感染途径。根据分离到的病毒特性 。
Isolation,Identification and Properties of Gosling New Type viral enteritis Virus (NGVEV) are reported in this paper.26 strains NGVEV were isolated from the parenchymal organs and intestines of the goslings with the clinical symptons and pathologic changes quite similiar to Gosling Plague by cell plague colone technology in duck embryo fibroblast monolayer cells.The antigenicity of 26 strains NGVEV was just the same.NGVEV showed round and ellipse,had no envelope,70～90 nm in diameter;could not agglutinate the red cells of the chick,duck,geese,pigeon,cattle,buffalo and pig;not sensitive to chloroform;its pathogenicity was not influenced at -15℃ for 36 months,0℃ for 20 months,37℃ for 45 days,45℃ for 48 h,56℃ for 5 h,60℃ for 1 h;but 80℃ for 5 min and boilling(96℃) for 10 s kill NGVEV.NGVEV existed in the band of 1 32 g/cm 3 in CsCl,nucleic acid was DNA.Duck Plague antisera and Gosling Plague antisera failed to neutralize NGVEV,NGVEV antisera failed to neutralize Duck Plague Virus(DPV) and Gosling Plague Virus(GPV)also.No influence to the NGVEV pathogenicity was observed in pH2 0～9 0 The virus could infect goslings by oral route,drop in eyes,drop in nose,subcutaneous injection,intramuscular injection,intravenous injection;and oral route was the best infectious way.According to the characteristics of NGVEV,it is classed as a new adenovirus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2000年第6期548-556,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目资助! (39970 5 6 1)
四川省跨世纪人才基金 !(970 0 0 0 2 40 0 0 6 )
关键词
雏鹅
新型病毒性肠炎
腺病毒
分离
鉴定
Isolation
Identification
Property
Gosling New Type viral enteritis Virus
