摘要
抗体筛选阳性克隆 ,用EcoRI酶切回收 3 6kb外源片段 ,测序。序列用大肠杆菌密码进行读框分析 ,至少有 6个开放阅读框 ,而在MG可能分为 3个开放阅读框。同源性分析 ,发现序列的某些碱基主要与肺炎支原体、生殖支原体、山羊支原体和莱氏无胆甾原体的某些地方同源性高 ,没有同其他细菌或病毒有同源性的地方 。
The DNA of recombinant phage was digested with restriction endonuclease of EcoRI.A 3 6 kb fragment was obtained.The 3 6 kb fragment was ligated with EcoRI digested and dephosphorylated pGEM 3ZF and sequenced.There were at least 6 open reading frames in the fragment based on sequence analysis with the standard codon of E.coli.The fragment had probably 3 open reading frames in the genome of MG.Computer analysis revealed homology,to some extent,between the 3 6 kb fragment and some regions of genome in Mycoplasma pneumoniae,M.genitalium,M.capricolum and Acholeplasma laidlawii.There was no homology between the 3 6 kb fragment and genome of bacteria or virus,this shows that the 3 6 kb fragment is exactly a new gene fragment.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2000年第6期543-547,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
鸡毒支原体
抗原
核苷酸序列
基因克隆
Mycoplasma gallisepticum
Gene
Antigen
Necleotide sequence
