摘要
目的:观察聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞修复大鼠坐骨神经缺损的效果。方法:将24只SD大鼠随机分为4组,制备右侧坐骨神经5mm缺损模型,A组聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞移植组;B组聚己内酯神经导管复合骨髓间充质干细胞移植组;C组壳聚糖神经导管复合骨髓间充质干细胞移植组;D组自体神经移植组。术后每2周进行坐骨神经功能指数检测,12周时行电生理、腓肠肌湿重恢复率、组织学观察和免疫组织化学检测。结果:坐骨神经功能指数显示,A组运动功能恢复速度较B、C组快,但比D组慢。A组电生理和腓肠肌湿重恢复率的检测结果与C、D组相比无统计学意义(P>0.05),但优于B组(P<0.05)。组织学观察,A组再生神经纤维排列密集。S-100免疫组织化学结果表明A组有大量雪旺细胞增生。结论:聚己内酯/壳聚糖神经导管复合骨髓间充质干细胞能够促进周围神经损伤修复,效果与壳聚糖神经导管、自体神经相同,优于聚己内酯神经导管。
Objective：To observe the effects of polycaprolactone/chitosan（PCL/CS）nerve conduit combined with bone marrow mesenehymal stem cells （BMSCs）for repairing sciatic nerve defect in rats. Methods：Twenty four SD rats were made a model of 5mm fight sciatic nerve defect in length and were randomly divided into four groups,group A： PCL/CS nerve conduit combined with BMSCs transplantation; group B： PCL nerve conduit combined with BMSCs transplantation;group C：CS nerve conduit combined with BMSCs transplantation;group D： autograft nerve. Sciatic function index （SFI）was evaluated every 2 weeks after surgery and tests of electrophysiology, the weight ratio of the gastrocnemius muscles, histological observation and immunohistochemistry were performed at 12 weeks postimplantation. Results：The recovery rate of motor function of group A was higher in comparison to group B and C but lower than that of group D as revealed by an SFI score. Compared the test results of electrophysiology and the weight ratio of the gastrocnemius muscles in group A with that in group C and D, there were not statistically significant （ P 〉 0.05 ）, but that in group A better than that in group B （ P 〈 0.05 ）. Histological observation ： the regenerative nerve of group A lined up intensively. S-100 protein immunohistochemistry showed there were much more regenerating Schwann cells in group A. Conclusion： PCL/CS nerve conduit combined with BMSCs can promote peripheral nerve injury repair and its result is the same as CS nerve conduit and autograft nerve but better than PCL nerve conduit.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2014年第2期34-38,共5页
China Biotechnology
基金
辽宁省高等学校优秀人才支持计划(LJQ2013090)资助项目
关键词
周围神经再生
神经导管
聚己内酯
壳聚糖
骨髓间充质干细胞
Peripheral nerve regeneration Nerve conduit Polycaprolactone Chitosan Bone marrow mesenchymal stem cells
