摘要
为了解2012年上海地区副溶血性弧菌人源株和食源株的优势血清型及其毒力基因携带状况，本研究收集了2012年从上海市15个区（县）腹泻患者和食品监测中分离的副溶血性弧菌株，进行血清分型，并采用聚合酶链反应（PCR）检测 tdh和trh基因。结果显示，854株副溶血性弧菌中，88．1％为血清可分型，89．8％为产毒株。O3∶K6、O4∶K8、O1∶K25、O4∶K68、O4∶K9、O1∶K36、O3∶K29为上海地区可分型人源株的优势血清型（93．8％），其中O3∶K6最多，达56．2％。副溶血性弧菌全部分离株的月份分布显示出聚集趋势，7～8月为高峰期。O4∶K9和O1∶K36血清型菌株的月份分布与其他优势血清型菌株不同，未表现出明显聚集趋势。食源株无明显优势血清型，且与人源株分布不同。人源株产毒株构成（95．6％）高于食源株（5．5％）。人源株优势血清型产毒株构成（99．9％）高于非优势血清型（71．1％）。血清可分型人源株的 tdh携带率（97．5％）高于不可分型人源株（67．6％），血清可分型人源株的 trh携带率（0．8％）低于不可分型人源株（42．6％）。结果提示，副溶血性弧菌血清型分布与历史数据相比变化较大，血清型与毒力基因携带呈一定程度关联，且人源株与食源株在血清型和毒力基因携带上具有分离现象。因此，在副溶血性弧菌的监测与检测中应充分考虑血清分型和毒力基因的重要性。
The main purpose of the research is to define serotype distribution and virulence factors of Vibrio parahaemolyticus （V . parahaemolyticus） isolates in Shanghai ,2012 .A total of 854 isolated strains collected through the diarrhea surveillance system from 15 district Centers for Disease Control and Prevention （739 isolates） ,food surveillance （49 strains） and suspected food poisoning cases （66 strains） were subjected to the assays for O∶ K serotypes and a polymerase chain reaction （PCR ）-based detection for tdh and （or ） trh genes respectively .88 .1% of the tested isolates could be serotyped with both O and K antisera ,and 89 .8%of the strains were positive for tdh and （or ） trh genes . Among the serotypeable isolates from diarrhea cases ,the positive rates for O3∶K6 ,O4∶K8 ,O1∶K25 ,O4∶K68 ,O4∶K9 ,O1∶K36 and O3∶K29 were 56 .2% ,13 .5% ,6 .8% ,7 .0% ,4 .5% ,2 .9% and 2 .9% respectively .O3 ∶ K6 was the most common serotype in Shanghai .The serotype distribution in food isolates was different from that in diarrhea isolates . The proportion of virulent strains in the diarrhea isolates （95 .6% ） was higher than that in the food isolates （5 .5% ） .Among diarrhea isolates , the proportion of virulent strains in the most common serotypes was higher than that in the other serotypes （71 .1% ） .The tdh detection rate in the serotypeable （97 .5% ） was higher than that in the unserotypeable （67 .6% ） ,but the trh detection rate in the serotypeable （0 .8% ） was lower than that in the unserotypeable （42 .6% ） .The results hint that the serotype constitute is different from that of historical data ,and tdh （ trh） carrying or not is likely to be related to serotypes .The isolates from diarrhea cases and food show separation phenomena in serotypes and virulent factors ,indicating the importance of serotyping and virulence factor detection in surveillance and detection of V . parahaemolyticus .
出处
《微生物与感染》
2014年第1期37-42,共6页
Journal of Microbes and Infections
基金
上海市卫生局青年科研项目(2010Y105)
上海市卫生局资助(12GWZX0801)
