摘要
目的探讨乙酰肝素酶(HPA)、蛋白激酶2β(CK2β)、缺氧诱导因子-1α(HIF-1α)在结肠癌中的表达及与其临床特征之间的关系。方法采用免疫组织化学染色技术Super-Vision法检测59例结肠癌组织标本及20例慢性结肠炎组织标本中HPA、CK2β及HIF-1α的表达水平。结果 HPA、CK2β与HIF-1α在结肠癌组织中的表达水平均高于其在慢性结肠炎组织,差异均有统计学意义(P<0.05);HPA、CK2β与HIF-1α的表达与临床分期和治疗后有无复发或转移有关(P<0.05),而与患者的性别无关(P>0.05);HIF-1α的表达与结肠癌患者的年龄密切相关(P<0.01),而HPA、CK2β则无相关性(P>0.05)。结论 HPA、CK2β和HIF-1α参与了结肠癌的发生、发展及转移。
Objective To investigate the expression of heparanase （HPA）, protein kinase CK2β （CK2β） and hy- poxia inducible factor-1α （HIF-1α） genes and their correlation with the clinical characteristics of colon cancer. Methods HPA, CK2β and HIF-lot expressions were detected with Super-Vision immunohistochemical assay on the tissues derived from 59 patients with colon cancer and 20 with chronic colitis. Results The expressions of HPA, CK2β and HIF-1α in colon cancer tissue were significantly heightened than those in chronic colitis tissues （all P〈 0.05）. The expressions of HPA, CK2β and HIF-1α were significantly related to the clinical stages and recurrence or metastasis following chemotherapy （both P〈O.05）, but not the gender （P〉0.05）. The expression of HIF-1α, but not HPA and CK2β （both P〉0.05）, was significantly related to the age in patients with colon cancer （P〈O.05）. Conclusion HPA, CK2β and HIF-1α are involved in the pathogenesis and metastasis of colon cancer.
出处
《中国药物与临床》
CAS
2014年第3期296-299,共4页
Chinese Remedies & Clinics
基金
广东省汕头市重点科技计划项目(2011-46-19)
