摘要
为了解猪繁殖与呼吸综合征病毒(PRRSV)流行株的变异特点,本试验从广东省疑似猪繁殖与呼吸综合征发病猪场采集的血清中分离到2株PRRSV,经RT-PCR检测为阳性,通过Marc-145细胞进行传代,能产生明显细胞病变。2株病毒分别命名为LZ-GD和LB-GD株PRRSV。对2株病毒的ORF5和Nsp2高变区进行序列测定和进化分析,结果显示,LZ-GD和LB-GD株的ORF5核苷酸序列与欧洲型代表株Lelystad株的同源性相对较远,分别仅为63.5%和63.8%,与美洲株经典毒株VR-2332的同源性分别为88.7%和89.1%,与中国高致病美洲毒株JXA-1的同源性分别为99.2%和99.3%。在分离病毒的Nsp2上存在30个氨基酸的不连续缺失,与中国高致病性变异株JXA-1的缺失位置一致,且同源性为99.1%和98.9%,结果表明分离的2株PRRSV均属于美洲型的变异株PRRSV。
Two strains of porcine reproductive and respiratory syndrome viruses （PRRSV） were isolated from serum of some pig farms in Guangdong province and showed PRRSV positive in RT-PCR testing. The two viruses could passage stably and cause typical cenotaphic effect, they were named as LZ-GD and LB-GD. The analysis of variable region sequences of ORF5 and Nsp2 of the two viruses showed that LZ-GD and LB-GD strains were far to Europe strain Lelystad, the homology of nucle- ar nucleotide sequence were 63.5~ and 63.8%0, respectively, with classic American strain VR-2332 were 88.7G and 89.1%, respectively, and that with highly pathogenic JXA-1 strain were 99.2% and 99.3%, respectively. There were 30 amino acids deletion in Nsp2. It shared the deletion with JXA-1, HUN4 and other pathogenic variant. Thus, the two strains of PRRSV be longed to highly pathogenic American type.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第3期33-38,共6页
China Animal Husbandry & Veterinary Medicine
基金
现代农业产业技术体系建设专项资金(CARS-36)
关键词
猪繁殖与呼吸综合征病毒
分离鉴定
进化分析
porcine reproductive and respiratory syndrome virus （PRRSV）
isolation and identification
genetic variation analysis
