摘要
目的了解耐碳青霉烯类鲍曼不动杆菌中插入序列ISAba1的存在对碳青霉烯酶基因表达的影响。方法筛选32株碳青霉烯类耐药的鲍曼不动杆菌,运用PCR技术检测OXA-23、OXA-24、OXA-51和OXA-58型酶基因及插入序列ISAba1;RT-PCR方法检测ISAba1下游基因的mRNA的相对表达水平。结果 OXA-51型基因的检出率为100%,OXA-23型基因为96.9%。其中23株OXA-23型基因、18株OXA-51型基因上游均存在插入序列。RT-PCR结果显示上游存在ISAba1时,OXA-23和OXA-51型基因相对表达水平分别为1.6471±0.6560和1.3209±0.3678,与不存在插入序列的相应基因表达水平相比,二者差异均有统计学意义(P<0.001)。结论 OXA-23和OXA-51型基因是本组试验菌的主要碳青霉烯酶基因型,ISAba1序列的插入可引起下游碳青霉烯酶基因高表达。
Objective To investigate the effect of the insert sequence ISAba1 on the expression of carbapenemase gene. Methods Of the 32 carbapenem-resistant Acinetobacter baumannii, PCR was used to detect the OXA-23, OXA-24, OXA-51, OXA-58 enzyme gene and insert sequence ISAba1; RT-PCR was used to detect the relative expression of mRNA of the ISAba1 downstream gene. Results The detection rate of OXA-5i and OXA-23 was 100% and 96.9%, respectively. Among them, 23 strains blaoxA.23 genes, 18 strains blaoXA.5l genes upstream present the insert sequence. RT-PCR results showed that when the upstream present ISAba1, the level of blaoXA.23 and blaoxA.51 expression was 1.6471±0.6560 and 1.3209±0.3678, respectively. And compared with no ISAbai, the differences both had statistical significance（P〈0.001）. Conclusion blaoXA.23 and blaoxA.51 gene was the main carbapenemase genotype in this group;ISAba1 sequence can cause high expression of the downstream carbapenemase gene.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2014年第3期229-233,共5页
Chinese Journal of Antibiotics
基金
国家自然科学基金资助项目(No.81373454)
四川省重点科技资助项目(No.2013jy0065)
