摘要
目的：建立刺山柑种子中没食子酸和芦丁的含量测定方法。方法采用Phnomenex色谱柱（250 mm ＆#215;4．6 mm ，5μm），以乙腈-2 mL · L -1磷酸溶液为流动相，流速为1．0 mL · min-1，检测波长为254 nm ，梯度洗脱。结果没食子酸在1.4～14．0μg、芦丁在1.4～28．0μg范围内呈良好的线性关系，没食子酸平均回收率为99．9％，RSD为2．0％；芦丁平均回收率为100．6％， RSD为1．3％。结论 HPLC法测定没食子酸和芦丁的含量，方法简便可行，重复性和分离效果好，可为刺山柑的进一步开发利用及质量控制提供依据。
Objective To determine of the contents of gallic acid and rutin in Capparis spinosa L .seeds .Methods The separation was performed on a Phnomennex ODS（250 mm ＆#215; 4 .6 mm ,5 μm） chromatographic column ,with acetonitrile-2 mL · L -1 phosph-pric acid solution as the mobile phase .The flow rate was 1 .0 mL ·min-1 ,the detection wavelength was 254 nm ,and the column temperature was 30 ℃ .Results The linear ranges of gallic acid and rutin were 1 .4-14 .0μg ,1 .4-28 .0μg with the correlation coef-ficient 0.999 5（n=6） ,0 .999 7（n=7） ,and the average recoveries were 99 .9% （RSD=2 .0% ） ,100 .6% （RSD=1 .3% ） ,respective-ly .Conclusion The method is simple and reproducible ,and could be used for C .spinosa L .further development ,utilization and quality control .
出处
《西北药学杂志》
CAS
2014年第2期117-119,共3页
Northwest Pharmaceutical Journal
基金
国家自然科学基金资助项目(编号:30561090)
