摘要
目的探讨Survivin、HPV16-E7蛋白、血管内皮生长因子(VEGF)在宫颈鳞癌及宫颈上皮内瘤样病变组织中的表达及在宫颈癌发生、发展中的作用。方法采用免疫组化SP法检测50例宫颈鳞癌、90例宫颈上皮内瘤变(其中CINⅠ、CINⅡ、CINⅢ各为30例)中Survivin、HPV16-E7蛋白及VEGF的表达,另取正常宫颈者20例为对照组。结果 Survivin、HPV16-E7蛋白及VEGF在宫颈鳞癌及宫颈上皮内瘤变(CINⅡ、CINⅢ)中的表达均随宫颈病变而呈递增趋势(P<0.05);Survivin的高表达与分化程度、临床分期及淋巴结转移有关(P<0.05);VEGF的高表达与分化程度、临床分期及淋巴结转移有关(P<0.05);HPV16-E7蛋白的高表达与分化程度、临床分期及淋巴结转移无关(P>0.05)。Survivin与HPV16-E7蛋白和VEGF的表达间存在正相关(r=0.417,P<0.01;r=0.378,P<0.01)。结论 Survivin、HPV16-E7蛋白及VEGF与宫颈癌的发生、发展密切相关。
Objective To explore the expression of survivin,HPV16-E7 protein and VEGF in tissue of cervical squamors cell carcinoma and cervical intraepithelial neoplasia and the effect during generation, development of cervical carcinoma. Methods Tile expression of survivin,HPV16-E7 protein and VEGF in 50 cases of cervical intraepithelial neoplasia and in 90 cases of cervical squamors cell carcinoma,including CIN I ,CIN Ⅱ ,CIN Ⅲ, respectively of 30 cases, were examined by SP immuohistochchemical method.Another 20 cases of normal cervical tissues as a control. Results Expression of survivin,HPV16-E7 protein and VEGF in cervical squamors cell carcinoma,including CIN 11 and CIN m, and in cervical intraepithelial neoplasia showed increasing trend （P〈0.05）. High expression of survivin was associated with tumor differentiation,clinical stage and lymph node metastasis （/9〈0.05）. High expression of VEGF was associated with tumor differentiation,clinical stage and lymph node metastasis（P〈0.05）. High expression of HPV16-E7 protein was not related to clinical stage,tumor differentiation and lymph node metastasis （P〉0.05）.Expression of survivin was posi- tively correlated with the expression of HPV16-E7 protein and VEGF （r=0.417, P〈0.01 ;r=0.378, P〈0.01）. Conclusion Survivin,HPV16-E7 and VEGF are closely related to the generation, development of cervical carcinoma.
出处
《中国现代医生》
2014年第8期101-103,107,共4页
China Modern Doctor
