摘要
雷公藤（Tripterygium wilfordii）不仅是一种药用植物，还是一种有毒植物，在民间作为杀虫植物，有“菜虫药”之称，其主要活性成分雷公藤生物碱和雷公藤甲素既有杀虫活性又有医用活性（李琰等，2012；Brinker et al．，2005；Liu，2011），临床上常用来治疗风湿性关节炎、肿瘤、免疫抑制性疾病（仲剑等，2009；Johnson et al．，2011；Huang et al．，2012）。
In this study, the effects of different concentration trace elements in NT medium on adventitious root growth, the accumulation of total alkaloid and triptolide of Tripterygium wilfordii were investigated. The results showed that the trace elements of Mn, Zn, Cu, Co, B, I and Mo influenced the adventitious root growth and the accumulation of total alkaloid and triptolide. The optimal NT medium trace elements for the growth of adventitious root were 2 times the concentration of MnSO4 ,with concentrations of ZnSO4 ,CuSO4, CoC12, H3 BO3 and KI maintained unchanged and 2 times concentrations of Na, MoQ. As for the accumulation of total alkaloids, the concentration of KI had no impact on the accumulation of total alkaloids, 2 times the concentration of CoCI2 and CuSO4 , removal of H3 BO3 and Na2 MoO4 , and 4 times concentrations of MnSO4 and ZnSO4 were suitable. In NT medium, 2 times the concentration of ZnSO4 and CuSO4 , removal of H3BO3 , Na2 MoQ and COC12, and 4 times concentrations of MnSQ and KI were optimal for the accumulation of triptolide.
出处
《林业科学》
CAS
CSCD
北大核心
2014年第2期134-138,共5页
Scientia Silvae Sinicae
基金
公益性行业(农业)科研专项(200903052)
中央高校基本科研业务费专项(QN2011110)
关键词
雷公藤
不定根
组织培养
微量元素
次生代谢产物
Tripterygium wilfordii
adventitious root
tissue culture
trace elements
secondary metabolites
