摘要
目的:应用基因芯片技术研究心房颤动(房颤)患者心房组织中miRNA的表达谱,分析差异表达的miRNA,为进一步研究miRNA在房颤发生、发展中的作用奠定基础。方法:采用miRNA基因芯片技术检测房颤患者和非房颤患者心房组织样本中miRNA的表达水平;采用实时定量PCR(quantitative real time PCR,qRT PCR)对部分差异表达的miRNA进行验证。结果:MiRNA基因芯片分析结果显示,与非房颤组织相比,房颤组织中差异表达的miRNA共有26个,其中16个miRNA表达上调,10个miRNA表达下调。qRT PCR验证结果与芯片结果相一致。结论:MiRNA在房颤患者心房组织中存在差异性表达。
Objective： To analyze the differentially expressed microRNAs （miRNA） in atrial tissues from patients with atrial fibrillation by gene chip technology and to lay a foundation for studing its role in the development of atrial fibrillation. Methods： The miRNA gene chip was used to detect the miRNA expression level in patients with atrial fibrillation and without atrial fibrillation. Quantitative real-time PCR （qRT-PCR） was used to verifythe differential expression of microRNAs.Results： q-he results of miRNA gene chip showed that there were 26 differentially expressed miRNAs in the atrial fibrillation group compared with the non-atrial fibrillation group. Among them, the expression levels of 16 miRNAs were up-regulated while 10 of them were down-regulated. The result of miRNA gene chip was in consistent with the result of qRT-PCR. Conclusion： The differential expression of miRNAs exists in the atrial tissues of patients with atrial fibrillation.
出处
《临床与病理杂志》
CAS
2014年第1期29-33,共5页
Journal of Clinical and Pathological Research
基金
国家自然科学基金(30900625/H0205)
衡阳市科技厅科研项目(2012KS13)~~
