摘要
利用三种发光波长的ZnS基纳米材料（ZnS、ZnS：Cu和ZnS：Mn）作为荧光标记物进行人免疫球蛋白（IgG）分子的免疫检测，X射线衍射（XRD）表明，过渡金属离子掺杂会导致ZnS纳米晶的结晶化尺寸减小；荧光光谱显示，ZnS、ZnS：Cu和ZnS：Mn纳米材料的发光波长分别为430nm、560nm和590nm。利用羊抗人IgG作为捕获抗体，分别制备免疫检测金基底和ZnS基荧光探针，分别进行空白实验、加入待测物人IgG实验，表明具有很好的检测选择性。
ZnS based on nanomaterials （ZnS,ZnS：Cu and ZnS：Mn） with three different kinds of emission wavelength were used as a fluorescent marker for human immunoglobulin （IgG） immune molecules detection. X ray diffraction （XRD） results showed that the transition metal ion doping leads to the size of ZnS nanoparticles decreased. Fluorescence spectroscopy results show that the wavelength of as-prepared ZnS,ZnS：Cu and ZnS：Mn nanomaterials were 430 nm,560 nm and 590 nm, respectively. Using goat anti-human IgG capture antibody immunoassay,gold substrate and various of ZnS based on fluorescent probe were prepared. Based on blank experiment,fluorescent probes were added to blank and analyte human IgG experiments. The result indicated we obtain the examination has the very good selectivity.
基金
基金项目：国家自然科学基金项目（61006065、61076039、61204065、61205193、61307045）,高等学校博士学科点专项科研基金项目（20102216110001、20102216120002、20112216120005）,吉林省自然科学基金项目（20101546）,吉林省科技发展计划项目（20121816、201201116）,吉林省教育厅项目（2011JYT05、2011JYT10、2011JYT11）.高功率半导体激光国家重点实验室基金项目（No.9140C310101120C031115）,长春市国际科技合作计划项目（2010CC02）.吉林农业大学科研启动基金项目（201238）
关键词
ZNS
多色荧光
免疫检测
ZnS
multicolor fluorescence
immunological detection
