摘要
目的：建立叠鞘石斛中联苄类化合物的含量测定方法。方法：以石斛酚为对照品，未加显色剂条件下，建立紫外可见分光光度法测定叠鞘石斛中联苄类化合物的含量。结果：确定最佳吸收波长280nm，石斛酚在浓度4．88-58．56μLg／mL范围内呈良好线性关系（r=0．9998），平均加样回收率为97．39％，RSD=2．97％。结论：该方法准确、简单、快捷，重现性好，适用于叠鞘石斛中联苄类化合物含量的测定。
Objective： To establish the content determination method for bibenzyl compounds in Dendrobinm denneanum. Methods： Under the condition of not adding chromogenic agent, using ultraviolet and visible spectmphotometfic determi- nation to detect the content of bibenzyl compounds with gigantol as the standard substance. Results： The best absorption wavelength was 280 nm. Gigantol had a liner relationship with the absorbance in the range of 4. 88 - 58. 56 p.g/mL （ r = 0. 9998 ） . The average re- covery for gigantol was 97. 39%, RSD was 2.97%. Conclusion： The method is simple, sensitive, accurate and can be used for bibenzyl compounds determination in Dendrobinm denneanum.
出处
《成都中医药大学学报》
2014年第1期7-10,共4页
Journal of Chengdu University of Traditional Chinese Medicine
基金
科技部"十二五"支撑计划项目(编号:2011BAI13BO2-8)
关键词
叠鞘石斛
石斛酚
联苄类化合物
含量测定
紫外可见分光光度法
Dendrobinm denneanum
gigantol
bibenzyl compounds
determination of contents
UV-visible spectrophotometry
