摘要
为了利用诱变技术快速创造变异类型丰富的油菜新材料用于油菜遗传改良,分别用0.4%、0.8%、1.0%和1.2%的EMS(甲基磺酸乙酯)溶液对甘蓝型油菜中双9号种子进行诱变处理。结果发现:(1)1.0%的EMS溶液是处理中双9号干种子的最适浓度,4种不同浓度处理M2表型变异率依次为11.26%、14.82%、27.19%和12.38%;(2)EMS诱变具有器官特异性;(3)M2突变体库中筛选到3株苯磺隆抗性突变体,突变率约为10-4。现已创建了包括子叶、叶片、花器、株型、角果等多器官变异类型的突变体库,为油菜遗传研究提供了丰富的种质资源。
To enhance rapeseed germplasm and improve rapeseed variety with resistance to tribenuron - methyl, seeds of Zhongshuang 9 （Brassica napus） were treated with 0.4% , 0.8% , 1.0% and 1.2% of EMS（ethyl methansulfonate） solution respectively. Results showed that （ 1 ） 1.0% of EMS was optional for mutagenesis and the phenotype variation in four M2 populations were 11.26% , 14.82% , 27.19% and 12.38% respectively. （2） Different concentrations of EMS had different effects on organs. （3） Three mutants with resistance to tribenuron methyl were with mutation frequency about 0. 000 1. The M2 mutant library, including mutants of cotyledons, leaves, flowers, plant -types, siliques, was available for genetic studies and breeding in rapeseed.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2014年第1期25-31,共7页
Chinese Journal of Oil Crop Sciences
基金
国家现代农业产业技术体系建设专项资金(CARS-13)
"十一五"国家科技支撑计划(2010BAD01B02)
陕西省科技统筹项目(K332021204)
西北农林科技大学拔尖人才支持计划
关键词
甘蓝型油菜
EMS
诱变
苯磺隆
抗除草剂突变体
Brassica napus L.
Ethyl metbansulfonate
Mutation
Tribenuron - methyl
Herbicide - resistantmutant
