摘要
目的 探讨癌胚抗原相关黏附分子1（CEACAMl）在胃癌中的表达与微血管生成的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（SP）法检测179例胃癌及40例癌旁非肿瘤胃组织中CEACAM1、CD34的表达.结果 CEACAM1在癌旁非肿瘤胃组织中并无阳性表达.174例胃腺癌标本均有CEACAM1表达,69.0％呈高表达,高、低表达CEACAMI标本中CD34-微血管密度（MVD）分别为（57.32±6.56）、（44.26 ±7.25）个/视野,两者差异有统计学意义（P＜0.05）.CEACAM1高表达及CD34-MVD分别与肿瘤分化程度、淋巴结转移和TNM分期密切相关（P＜0.05）.结论 胃癌中CEACAM1的表达上调可能参与胃癌微血管生成,提高其侵袭和转移能力.
Objective To investigate the relationship between carcinoembryonic antigen-related cell adhesion molecule 1 （CEACAM1） expression and angiogenesis in gastric cancer.Methods CEACAM1 and CD34 expression was detected by immunohistochemistry （IHC） in 174 cases of gastric cancer and 40 cases of nonneoplastic gastric tissues.Results No CEACAM1 expression was found in nonneoplastic gastric mucosa tissues.CEACAM1 expression was classfied as high and low （69.0％ vs.31.0％）,the CD34-microvessel density （MVD） expression between them was significantly different （57.32 ± 6.56 vs.44.26 ± 7.25,P ＜ 0.05）.Coexpression of CEACAM1 and CD34-MVD was elevated and significantly different from nonneoplastic to neoplastic lesions（P ＜0.05）.Moreover,high CEACAM1 expression and CD34-MVD were closely associated with degree of tumor differentiation,nodal metastasis and TNM staging.Conclusion Up-regulating CEACAM1 may participate in tumor angiogenesis,and promote its capability of invasion and metastasis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第3期522-523,共2页
Chinese Journal of Experimental Surgery
关键词
胃癌
癌胚抗原相关黏附分子l
CD34
免疫组织化学技术
Gastric cancer
Carcinoembryonic antigen-related cell adhesion molecule 1
CD34
Immunohistochemistry
