期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

亚硒酸钠对延边奶山羊乳腺上皮细胞过氧化氢所致氧化损伤的保护作用 被引量：2

在线阅读 下载PDF
导出
摘要 研究了亚硒酸钠对过氧化氢导致的延边奶山羊乳腺上皮细胞氧化损伤的保护作用。将延边奶山羊乳腺上皮细胞分为3组:正常组(对照组1)、过氧化氢损伤组(对照组2)、亚硒酸钠保护组。MTT方法检测细胞活力,Hoechst33342/PI双染、Giemsa染色法检测细胞凋亡情况,DHE染色法检测细胞内ROS含量。结果表明,与对照组2相比,浓度为0.25、0.5、1.0μg/mL的亚硒酸钠保护组的延边奶山羊乳腺上皮细胞活力上升,细胞凋亡数目减少,活性氧含量减少。其中以0.5μg/mL亚硒酸钠处理组效果最显著。说明0.5μg/mL的亚硒酸钠可以减少过氧化氢所致的延边奶山羊乳腺上皮细胞的氧化损伤,提高细胞活力,抑制细胞凋亡,减少细胞内ROS含量。表明亚硒酸钠对延边奶山羊乳腺上皮细胞过氧化氢所致氧化损伤具有保护作用。
作者 孙婧陶 刘佳丽 张宝修 茹李波 李钟淑 方南洙
机构地区 延边大学农学院/动物遗传育种与繁殖实验室 吉林省安图县畜牧兽医局
出处 《江苏农业科学》 北大核心 2014年第2期155-158,共4页 Jiangsu Agricultural Sciences
基金 吉林省科技厅重点项目(编号:20100228)
关键词 亚硒酸钠 乳腺上皮细胞 氧化损伤 凋亡
分类号 S827.1 [农业科学—畜牧学]
  • 相关文献

参考文献31

  • 1Wiseman H, Halliwell B. Damage to DNA by reactive oxygen and ni?trogen species: role in inflammatory disease and progression to cancer[J]. BiochemicalJournal, 1996 ,313: 17 -29.
  • 2Hunt C R, SimJ E, Sullivan SJ , et al. Genomic instability and cata- lase gene amplification induced by chronic exposure to oxidative stress[J]. Cancer Research,1998,58(17) :3986 -3992.
  • 3Mills EM, Takeda K, Yu Z X, et s!. Nerve growth factor treatment prevents the increase in superoxide produced by epidenns! growth fac?tor in PC12 cells[J].Journal of Biologics! Chemistry, 1998,273 (35) :22165 - 22168.
  • 4Kajta M. Apoptosis in the centrs! nervous system: Mechanisms and protective strategies[J] . PolishJournal of Pharmacology, 2004 , 56 (6):689-700.
  • 5Kajta M, Lason W, Kupiec T. Effects of estrone on N - methyl - D - aspartic acid - and staurosporine - induced changes in caspase - 3 - like protease activity and lactate dehydrogenase - release: time - and tissue - dependent effects in neurons! primary cultures[J]. Neuro?science,2004,123(2) :515 -526.
  • 6Amstad P, Moret R, Cerutti P. Glutathione peroxidase compensates for the hypersensitivity of Cu , Zn - superoxide dismutase overproducers to oxidant stress[J].Journal of Biologics! Chemistry, 1994,269 ( 3 ) : 1606 -1609.
  • 7Czene S, Tiback M, Harms - Ringdahl M. pH - dependent DNA cleavage in penneabilized human fibroblasts[J]. BiochemJ, 1997 , 323:337 -34l.
  • 8TamaritJ, Cabiscol E, RosJ. Identification of the major oxidatively damaged proteins in Escherichia coli cells exposed to oxidative stress[J].Journal of Biologics! Chemistry,1998,273(5) :3027 -3032.
  • 9Toledano BJ, Bastien Y , Noya F , et s!. Platelet - activating factor ab?rogates apoptosis induced by cross - linking of the surface IgM recep?tor in a human B lymphoblastoid cell line[J].Journal of Immunolo?gy, 1997 , 158 (8) :3705 - 3715.
  • 10ZhangJ , Robinson D, Sslmon P. A novel function for selenium in bi?ologics! system: selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclons! antibody production[J] . Biotechnology and Bioengineering,2006 ,95 (6) : 1188 -1197.

二级参考文献17

  • 1FeiGAO,JingYI,JingQiYUAN,GuiYingSHI,XueMingTANG.The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species[J].Cell Research,2004,14(1):81-85. 被引量：21
  • 2张斌,夏作理,赵晓民,张和平.氧化应激模型的建立及其评价[J].中国临床康复,2006,10(44):112-114. 被引量：38
  • 3Janisch K M, Milde J, Schempp H, et al. Vitamin C, vitamin E and flavonoids[ J ]. Dev Ophthalmo1,2005 ,38 :59 - 69.
  • 4Karageuzyan K G. Oxidative stress in the molecular mechanism of pathogenesis at different diseased states of organism in clinics and experiment [ J ]. Current Drug Targets Inflammation and Allergy, 2005,4( 1 ) :85 -98.
  • 5Bellavite P. The superoxide - forming enzymatic system of phagocytes [ J ]. Free Radical Biology and Medicine, 1988,4 ( 4 ) : 225 - 261.
  • 6Giulian D, Vaea K, Corptrz M. Brain glia release factors with opposing actions upon neuronal survival[ J ]. The Journal of Neuroscience:the Official Journal of the Society for Neuroscience, 1993,13 ( 1 ) :29 - 37.
  • 7Richter C, Kass G E. Oxidative stress in mitochondria:its relationship to cellular Ca^2+ homeostasis, cell death, proliferation, and differentia- tion[ J]. Chemico - biological Interactions, 1991,77 ( 1 ) : 1 - 23.
  • 8Yu B P. Cellular defenses against damage from reactive oxygen species[ J ]. Physiological Reviews, 1994,74 ( 1 ) : 139 - 162.
  • 9Sun L K, Yoshii Y, Miyagi K. Cytotoxic effect through fas/hPO - l expression due to vitamin K in human glioma cells [ J ]. Journal of Neuro - oneology ,2000,47( 1 ) :31 - 38.
  • 10Farombi E O, M~ller P, Dragsted L O. Ex - vivo and in vitro protec- tive effects of kolaviron against oxygen - derived radical - induced DNA damage and oxidative stress in human lymphocytes and rat liver cells [ J ]. Cell Biology and Toxicology,2004,20 ( 2 ) : 71 - 82.

共引文献16

同被引文献38

引证文献2

二级引证文献11

江苏农业科学
2014年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部