摘要
目的研究Reelin对小鼠海马神经元多巴胺D1受体的调控及其是否依赖Reelin受体(ApoER2/VLDLR)发挥调控作用。方法原代分离培养新生C57BL/6J小鼠海马神经元,将培养5 d后的神经元随机分为6组:空白对照组、CR-50组(CR-50),EDTA组(EDTA),Reelin组(Reelin),Reelin拮抗组(Reelin+CR50)和Reelin受体拮抗组(Reelin+EDTA)。分别加入Reelin、CR-50、EDTA进行共培养,其后利用免疫荧光的方法观察Reelin受体ApoER2、VLDLR和多巴胺D1受体在海马神经元上的定位以及各组多巴胺D1受体的变化。结果 (1)Reelin受体ApoER2、VLDLR均与多巴胺D1受体在海马神经元上共定位;(2)Reelin拮抗组的多巴胺D1受体荧光强度(0.215±0.09)较Reelin组(0.663±0.15)减弱(P<0.01);(3)Reelin受体拮抗组的多巴胺D1受体荧光强度(0.687±0.11)与Reelin组(0.666±0.15)无明显差异(P>0.05)。结论Reelin可增强小鼠海马神经元上的多巴胺D1受体的表达,Reelin上调多巴胺D1受体的作用并不是依赖Reelin的经典受体ApoER2、VLDLR,其调控机制有待进一步研究。
Objective To investigate the regulation of dopamine D1 receptor by Reelin in mouse hippocampal neurons, and whether the regulatory effects are dependent on Reelin receptors （ApoER2/VLDLR）. Methods The neurons were cultrnred from the hippocampus of newborn C57BL/6J mice. The neurons cultured for 5 days were randomly divided into six groups, including control group, CR-50 group （CR-50）, EDTA group（EDTA）, reelin group （Reelin）, reelin antagonist group （Reelin＋CR-50）, reelin re- ceptor antagonist group （Reelin＋EDTA）. The recombinant Reelin, CR-50, EDTA were added and coineubated with hippocampal neu- rons for 1.5 hours, subsequently, the localization of ApoER2, VLDLR, dopamine D1 receptor in hippocampal neurons and the ex- pression of dopamine D1 receptor were examined by immunofluoreseence. Results （1） ApoER2, VLDLR were co-localized with dopamine D 1 receptor in hippocampal neurons. （2） Compared with the Reelin group （0.663±0.15 ）, the Dopamine D 1 receptor fluo- rescence intensity of reelin antagonist group （0.215±0.09） showed a significant decrease（P〈0.01 for both）. （3） Compared with the Reelin group （0.666±0.15）, the dopamine D1 receptor fluorescence intensity of reelin receptor antagonist group （0.687±0.11） did not change significantly. Conclusion Reelin increased dopamine D1 receptors in mouse hippocampal neurons, and the increasement is not dependent on Reelin receptors ApoER2, VLDLR. Its regulatory mechanism needs further study.
出处
《解剖学研究》
CAS
2014年第1期1-7,共7页
Anatomy Research
基金
国家自然科学基金项目(81273350)
